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Sample GSM82671 Query DataSets for GSM82671
Status Public on Sep 06, 2006
Title proband f, past exhaustive exercise
Sample type RNA
Source name white blood cells
Organism Homo sapiens
Characteristics male, healthy, no medicamentation
Extracted molecule total RNA
Extraction protocol 9ml blood were collected and erythrocyte lysis using Qiagen Erythrocyte lysis buffer was performed immediately. White blood cells were washed in icecold PBS two times. Cells were then lysed using Trizol (Invitrogen).Phase lock gel tubes (Eppendorf) were used to extract the RNA containing phase. Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
Label biotin
Label protocol Approximately 3 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description proband f, past exhaustive exercise, white blood cells, male, healthy, no medicamentation
Data processing GCOS 1.2 and GeneSpring 7.1 (Agilent)
Submission date Nov 10, 2005
Last update date Sep 06, 2006
Contact name Sandy Mosig
Phone +49-3641-934813
Fax +49-3641-933950
Organization name University Hospital Jena
Department Molecular Hemostaseology - Institute of Vascular Medicine
Street address Bachstrasse 18
City Jena
ZIP/Postal code 07743
Country Germany
Platform ID GPL571
Series (1)
GSE3606 Gene expression profiles in white blood cells in response to exercise

Data table header descriptions
ID_REF Affymetrix annotation numbers
VALUE GCOS 1.2 -calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 593.3 P 0.000581
AFFX-BioB-M_at 1049.4 P 0.000044
AFFX-BioB-3_at 487.1 P 0.001593
AFFX-BioC-5_at 1499.1 P 0.000095
AFFX-BioC-3_at 1329.5 P 0.000044
AFFX-BioDn-5_at 1448.2 P 0.000044
AFFX-BioDn-3_at 7186.8 P 0.00007
AFFX-CreX-5_at 18590.1 P 0.000052
AFFX-CreX-3_at 21779.1 P 0.000044
AFFX-DapX-5_at 42.3 P 0.046482
AFFX-DapX-M_at 10.5 A 0.659339
AFFX-DapX-3_at 14 A 0.852061
AFFX-LysX-5_at 4.7 A 0.824693
AFFX-LysX-M_at 43.7 A 0.58862
AFFX-LysX-3_at 2.9 A 0.672921
AFFX-PheX-5_at 3.5 A 0.945787
AFFX-PheX-M_at 3.7 A 0.932322
AFFX-PheX-3_at 9.6 A 0.794268
AFFX-ThrX-5_at 11.8 A 0.631562
AFFX-ThrX-M_at 9.2 A 0.724854

Total number of rows: 22277

Table truncated, full table size 592 Kbytes.

Supplementary data files not provided

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