|
Status |
Public on Nov 05, 2011 |
Title |
col096 |
Sample type |
RNA |
|
|
Source name |
Primary tumor resection
|
Organism |
Homo sapiens |
Characteristics |
disease status: AJCC stage II CRC tissue: primary tumor resection age at diagnosis: 61,91 Sex: m meta or recurrence within 3 years: no time to meta or recurrence: 1147
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using Trizol (Invitrogen) and purified with RNeasy (Qiagen) according to manufacturer’s protocol.
|
Label |
biotin
|
Label protocol |
RNA sample integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies). 4 µg of total RNA was used for cRNA synthesis and fragmented. Labeling was performed with One-Cycle cDNA synthesis Kit (Affymetrix) according to manufacturer’s protocol. Sample quality was checked on a Bioanalyzer prior and after fragmentation.
|
|
|
Hybridization protocol |
10 µg of labeled cRNA was hybridized to Affymetrix Human genome U133 Plus 2.0 arrays according to manufacturer’s protocol (Affymetrix).
|
Scan protocol |
Arrays were scanned with a GeneChip Scanner 3000 (Affymetrix) according to manufacturer’s protocol
|
Description |
COL096.CEL
|
Data processing |
Expression data (cel files) were normalized with the MAS5.0 algorithm (target signal = 100) using GCOS software (Affymetrix)
|
|
|
Submission date |
Oct 20, 2011 |
Last update date |
Mar 18, 2013 |
Contact name |
Richard Volckmann |
E-mail(s) |
r.volckmann@amsterdamumc.nl
|
Organization name |
Academic Medical Centre, University of Amsterdam
|
Department |
Department of Oncogenomics
|
Street address |
P.O. Box 22700
|
City |
Amsterdam |
ZIP/Postal code |
1100DE |
Country |
Netherlands |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE33113 |
AMC colon cancer AJCCII |
GSE33114 |
Methylation of Cancer Stem Cell-associated Wnt target genes predicts poor prognosis in colorectal cancer patients |
|