|
Status |
Public on Apr 10, 2024 |
Title |
HL-1 cells, control rep3 |
Sample type |
SRA |
|
|
Source name |
heart
|
Organism |
Mus musculus |
Characteristics |
tissue: heart cell line: HL-1 cells genotype: WT treatment: Control
|
Treatment protocol |
tachypacing
|
Growth protocol |
HL-1 cells were cultured with Claycomb Medium supplemented with 10 % FBS, 100 U/mL penicillin/streptomycin, 0.1 mM norepinephrine, and 2 mM L-glutamine.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was obtained using Qiagen RNA extraction kit RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacture's protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina Genome Analyzer II |
|
|
Description |
Sample3
|
Data processing |
CLC Genomics Workbench v 11.0.1 Sequence reads were trimmed for adaptor sequence/low quality sequence using CLC genomic benchword (paarameter-Quality limit: 0.05) Trimmed sequence reads were mapped to GrCM38/mm10 using CLC genomic benchwork (parameter-mismath cost:2 insertion cost:3 deletion cost:3 length fraction: 0.8 similartiy fraction: 0.8) Read count extraction and normalization were performed using CLC genomic benchwork Assembly: mm10 Supplementary files format and content: tab-delimited text file includes raw counts for each Sample Supplementary files format and content: tab-delimited text file includes RPKM values
|
|
|
Submission date |
Apr 05, 2024 |
Last update date |
Apr 10, 2024 |
Contact name |
Wenjing Chen |
E-mail(s) |
xifeng2024@outlook.com
|
Organization name |
Zhejiang University
|
Street address |
No.79 Qingchun Road
|
City |
Hangzhou |
ZIP/Postal code |
310000 |
Country |
China |
|
|
Platform ID |
GPL9250 |
Series (1) |
GSE263301 |
The electrophysiological functions of macrophages are involved in the development of atrial fibrillation |
|
Relations |
BioSample |
SAMN40763735 |
SRA |
SRX24172637 |