|
| Status |
Public on Apr 10, 2024 |
| Title |
HL-1 cells, control rep3 |
| Sample type |
SRA |
| |
|
| Source name |
heart
|
| Organism |
Mus musculus |
| Characteristics |
tissue: heart
cell line: HL-1 cells
genotype: WT
treatment: Control
|
| Treatment protocol |
tachypacing
|
| Growth protocol |
HL-1 cells were cultured with Claycomb Medium supplemented with 10 % FBS, 100 U/mL penicillin/streptomycin, 0.1 mM norepinephrine, and 2 mM L-glutamine.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was obtained using Qiagen RNA extraction kit
RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacture's protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
Sample3
|
| Data processing |
CLC Genomics Workbench v 11.0.1
Sequence reads were trimmed for adaptor sequence/low quality sequence using CLC genomic benchword (paarameter-Quality limit: 0.05)
Trimmed sequence reads were mapped to GrCM38/mm10 using CLC genomic benchwork (parameter-mismath cost:2 insertion cost:3 deletion cost:3 length fraction: 0.8 similartiy fraction: 0.8)
Read count extraction and normalization were performed using CLC genomic benchwork
Assembly: mm10
Supplementary files format and content: tab-delimited text file includes raw counts for each Sample
Supplementary files format and content: tab-delimited text file includes RPKM values
|
| |
|
| Submission date |
Apr 05, 2024 |
| Last update date |
Apr 10, 2024 |
| Contact name |
Wenjing Chen |
| E-mail(s) |
xifeng2024@outlook.com
|
| Organization name |
Zhejiang University
|
| Street address |
No.79 Qingchun Road
|
| City |
Hangzhou |
| ZIP/Postal code |
310000 |
| Country |
China |
| |
|
| Platform ID |
GPL9250 |
| Series (1) |
| GSE263301 |
The electrophysiological functions of macrophages are involved in the development of atrial fibrillation |
|
| Relations |
| BioSample |
SAMN40763735 |
| SRA |
SRX24172637 |
