|
| Status |
Public on Oct 13, 2011 |
| Title |
WT_epidermis_neonatal_biological_replicate_3 |
| Sample type |
RNA |
| |
|
| Source name |
ItgA6+ neonatal epidermis from mouse 3
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6 CBA mixed background
mouse: 3
genotype/variation: wildtype
sorted cell population: ItgA6+ neonatal epidermal keratinocytes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was purified from sorted cell populations using the Purelink Micro-to-Midi Total RNA Purification System Kit (Invitrogen), including an on-column Dnase treatment step
|
| Label |
Biotin
|
| Label protocol |
RNA amplification using the WT-Ovation Pico target prep v 1.0 protocol derived from the NuGE WT-Ovation Pico RNA Amplification system (WTO Pico UserGuide) and the NugEN FL-Ovation cDNA Biotin Module
|
| |
|
| Hybridization protocol |
Hybridization cocktail prepared using the NugEN Cocktail PICR v1.0 protocol derived from the NuGEN FL-Ovation cDNA Biotin Module v2 user guide (FLBv2 User Guide)
|
| Scan protocol |
Affymetric GeneChip scanner 3000 running GCOS software located the PICR
|
| Description |
ItgA6+ WT neonatal epidermal keratinocytes
|
| Data processing |
RMA normalisation (GeneSpring GX11). The expression calls were generated by java implementation (from The Paterson Institute for Cancer Research) of the MAS5.0 algorithm, as described in [Hubbel et al (2002) Bioinformatics 18, Liu et al (2002) Bioinformatics 18].
|
| |
|
| Submission date |
Oct 13, 2011 |
| Last update date |
Oct 14, 2011 |
| Contact name |
Kai Kretzschmar |
| E-mail(s) |
kk405@cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
Wellcome Trust Centre for Stem Cell Research
|
| Lab |
Watt Lab
|
| Street address |
Tennis Court Road
|
| City |
Cambridge |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB2 1QR |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE32966 |
Dermal reprogramming through epidermal activation of beta-catenin |
|
