|
| Status |
Public on Mar 18, 2024 |
| Title |
iWAT, estrogen treated group , Cold 7days, rep1 |
| Sample type |
SRA |
| |
|
| Source name |
inguinal white adipose tissue
|
| Organism |
Mus musculus |
| Characteristics |
tissue: inguinal white adipose tissue
treatment: Estrogen upon cold exposure
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol kit (Ambion). 1.3 ug of total RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-seq were prepared by Novogene company (China).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
E2_1
|
| Data processing |
Data Quality Control: filtering reads containing adapter or with low quality: Pastp
Mapping Reads to Reference Genome : HISAT2
Gene Expression Quantification : Feature Counts
Assembly: mm10
Supplementary files format and content: tab-delimited text file includes raw counts for each Sample
|
| |
|
| Submission date |
Mar 13, 2024 |
| Last update date |
Mar 18, 2024 |
| Contact name |
Jooman Park |
| E-mail(s) |
joomanp@uic.edu
|
| Organization name |
University of Illinois at Chicago
|
| Department |
Dept. of Physiology and Biophysics
|
| Lab |
Jiang Yuwei
|
| Street address |
909 S Wolcott Ave COMRB 1120
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60612 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE261481 |
Effect of estrogen on transcriptomic profile in iWAT of old aged mice |
|
| Relations |
| BioSample |
SAMN40436129 |
| SRA |
SRX23938178 |
