|
| Status |
Public on Nov 11, 2011 |
| Title |
Middle HSC 5 |
| Sample type |
RNA |
| |
|
| Source name |
Middle HSC
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: bone marrow
cell type: hematopoietic stem cell
age: 56
|
| Treatment protocol |
Mononuclear cells were prepared using Ficoll-Paque Plus (GE Healthcare), and either analyzed/sorted fresh or cryopreserved in 90% FBS, 10% DMSO in liquid nitrogen. Human CD34-positive cells were enriched using magnetic beads (Miltenyi Biotec, Germany; Stemcell Technologies, Vancouver, BC).
|
| Growth protocol |
Analyzed/sorted fresh or cryopreserved in 90% FBS, 10% DMSO in liquid nitrogen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol (Invitrogen) or Ambion RNA Isolation Kit (Applied Biosystems by Life Technologies, Carlsbad, CA), according to the manufacturer's protocols, and treated with DNaseI (Qiagen, Germany).
|
| Label |
biotin
|
| Label protocol |
All RNA samples were quantified with the RiboGreen RNA Quantitation Kit (Molecular Probes, Eugene, OR), subjected to reverse transcription, 2 consecutive rounds of linear amplification, and production and fragmentation of biotinylated cRNA (Affymetrix, Santa Clara, CA).
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedures
|
| Scan protocol |
Standard Affymetrix procedures
|
| Description |
Gene expression data from middle aged HSC
|
| Data processing |
The data were normalized using RMA.
|
| |
|
| Submission date |
Oct 10, 2011 |
| Last update date |
Nov 11, 2011 |
| Contact name |
Debashis Sahoo |
| E-mail(s) |
sahoo@stanford.edu
|
| Phone |
650-862-4736
|
| Fax |
(650) 736-2961
|
| URL |
http://www.stanford.edu/~sahoo
|
| Organization name |
Stanford University
|
| Department |
Pathology
|
| Lab |
Weissman lab
|
| Street address |
265 Campus Drive, Rm G3101B
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE32719 |
Expression data from human bone marrow hematopoietic stem cells |
|
