|
| Status |
Public on Apr 19, 2024 |
| Title |
Prostate cancer, Estradiol + Testosterone, rep 3 |
| Sample type |
SRA |
| |
|
| Source name |
Prostate
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Prostate
genotype: C57BL/6J PB-Cre4+/-;Ptenfl/fl
treatment: 17β-Estradiol and Testosterone (each 30 µg/g of mouse weight)
|
| Treatment protocol |
Mice of at least 24 weeks old where firstly castrated, and 72h later, they received sub-cutaneous injections of either vehicule (1:10 ethanol 96% / vegetable oil), Estradiol (30 µg/g of mouse weight) and/or Testosterone (30 µg/g of mouse weight). Estradiol will activate the estrogen receptor alpha (ERa), whereas Testosterone will activate the androgen receptor (AR).
|
| Growth protocol |
Thirty mice in total (16 C57BL/6J Ptenfl/fl [normal or WT] and 14 C57BL/6J PB-Cre4+/-;Ptenfl/fl [prostate cancer]) were bred and housed in a 12 hour light, 12h dark cycle at 22°C.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
24 hours after injections, mice prostates were harvested, and RNA was extracted and purified with the QIAGEN RNeasy Mini Kit.
mRNA library was generated using NEBNext Ultra II Directional RNA library prep kit.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
PCa_ET-3
|
| Data processing |
Trimming: Trim Galore/Cutadapt
Quality check: FastQC/MultiQC
Pseudoalignement: Kallisto
Differential analysis and normalization : tximport/DESeq2
Assembly: GRCm38.p6
Supplementary files format and content: normalized_counts.csv.gz: comma separated values containing normalized expression for each sample
Supplementary files format and content: raw_counts.csv.gz: comma separated values containing raw counts for each sample
|
| |
|
| Submission date |
Jan 30, 2024 |
| Last update date |
Apr 19, 2024 |
| Contact name |
Étienne Audet-Walsh |
| E-mail(s) |
etienne.audet-walsh@crchudequebec.ulaval.ca
|
| Organization name |
CRCHU de Québec
|
| Street address |
2705, boulevard Laurier
|
| City |
Québec |
| State/province |
Québec |
| ZIP/Postal code |
G1V 4G2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE254635 |
The reprogrammation of the estrogen and androgen responses in murine prostate cancer |
|
| Relations |
| BioSample |
SAMN39676980 |
| SRA |
SRX23454846 |
