|
| Status |
Public on May 22, 2024 |
| Title |
PHBI_004 |
| Sample type |
SRA |
| |
|
| Source name |
lung
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: lung
Sex: Female
condition: PH
ph subtype: IPAH
sequencingbatch: 2
age: 48
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by Zymo Direct-zol kit using on column DNAse treatment.
RNA libraries were preparing using TruSeq Stranded mRNA HT (RS-122-2103), dual index.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
| |
|
| Data processing |
Sequence reads were aligned to GENCODE human reference genome (v. 32) using HISAT2.
Transcripts were assembled and quantified using StringTie.
Transcript-level abundance estimates were imported and summarized into a counts matrix using tximport.
Assembly: GRCh38.p13
Supplementary files format and content: csv file containg counts estimated by tximport
|
| |
|
| Submission date |
Jan 30, 2024 |
| Last update date |
Jul 03, 2024 |
| Contact name |
Jason Hong |
| Organization name |
UCLA
|
| Street address |
10833 Le Conte Ave, 43-229 CHS
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL21290 |
| Series (1) |
| GSE254617 |
Integrative Multiomics in the Lung Reveals a Protective Role of Asporin in Pulmonary Arterial Hypertension. |
|
| Relations |
| BioSample |
SAMN39676698 |
| SRA |
SRX23453832 |
