|
| Status |
Public on Jan 10, 2024 |
| Title |
Breast Cancer 26 b |
| Sample type |
RNA |
| |
|
| Source name |
Breast tumor
|
| Organism |
Homo sapiens |
| Characteristics |
filename: A1764-009_b
plate_pos: H10
subject_id: 26
crna_conc_ng_per_ul: 1510
ratio_260_280: 2.28
ratio_260_230: 2.59
conc_ug_per_ul: 1.51
final_vol_ul_1: 22
crna_yield_ug: 33.22
crna_used_ug: 4
vol_crna_used_ul: 2.64900662251656
final_vol_ul_2: 22.5
rnase_free_water_ul: 19.8509933774834
batch: Batch1
cdna_conc_ng_per_ul: NA
cdna_yield_ug: NA
cdna_used_ug: NA
vol_cdna_used_ul: NA
dataset: FinHER
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from FFPE specimens using Qiagen's miRNeasy FFPE Kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to Affymetrix's standard protocols.
|
| |
|
| Hybridization protocol |
RNA extracted from FFPE specimens with Qiagen's miRNeasy FFPE Kit was processed with Affymetrix’s SensationPlus FFPE Amplification and 3'IVT Labeling Kit. The processed RNA was hybridized on the Affymetrix’s Human Genome U219 Array Plate (96 wells) using the GeneTitan instrument.
|
| Scan protocol |
The 96 well array plates were scanned using Affymetrix's automated GeneTitan system according to the manufacturer's instructions.
|
| Description |
Biotinylated cRNA was prepared according to Affymetrix's standard protocols
|
| Data processing |
The probe level intensities were background adjusted using the Robust multi-array analysis (RMA) method followed by quantile normalization as impletemented in the R/Bioconductor package affy.
|
| |
|
| Submission date |
Jan 10, 2024 |
| Last update date |
Jan 10, 2024 |
| Contact name |
Christos Sotiriou |
| E-mail(s) |
christos.sotiriou@hubruxelles.be
|
| Phone |
+32 2 541 34 57
|
| Organization name |
Institut Jules Bordet – Université Libre de Bruxelles (ULB)
|
| Lab |
Breast Cancer Translational Research Laboratory J.C. Heuson (ULB 290)
|
| Street address |
Rue Meylemeersch 90
|
| City |
Brussels |
| ZIP/Postal code |
1070 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL13667 |
| Series (1) |
| GSE47994 |
Tumor-infiltrating lymphocytes, prognosis and trastuzumab benefit in early-stage breast cancer patients from the FinHER trial |
|
