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Sample GSM798475 Query DataSets for GSM798475
Status Public on Apr 29, 2012
Title Treg WT rep3
Sample type RNA
Source name wild type Treg, replicate 3
Organism Mus musculus
Characteristics strain: C57BL/6
gender: Female
age: 6 weeks old
cell type: Treg
treatment: unstimulated
genotype: WT
Treatment protocol Stimulations were done with plate-coated anti-CD3(1mg/ml) and anti-CD28 (1mg/ml)for 13 hours.
Growth protocol Mice were maintained under specific pathogen-free conditions according to institutional guidelines under approved protocols in Kyoto University.
Extracted molecule total RNA
Extraction protocol Total RNAs were extracted with Qiagen RNeasy mini kit with on-column DNaseI treatment. RNA quality was assessed using Agilent 2100 Bioanalyzer according to manufacturer's instructions.
Label Cy3
Label protocol Cy3 labeled cRNA was produced using Agilent Low Input QuickAmp Labeling Kit according to manufacturer's protocol.
Hybridization protocol For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
Scan protocol Slide were washed and then scanned with an Agilent G2505B scanner
Data processing Data were obtained and processed with the Agilent Feature Extraction Software(v9.5). The resulting data were imported into Expressionist and quantile normalized intensities were exported.
Submission date Sep 19, 2011
Last update date Apr 29, 2012
Contact name Tasuku Honjo
Organization name Kyoto University
Department Department of Immunology and Genomic Medicine
Street address Yoshida Konoe-cho, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8501
Country Japan
Platform ID GPL7202
Series (1)
GSE32224 Comparison of gene expression in Wild type and T cell-specific conditional Trim28 KO in TCR stimulated and un-stimulated naive CD4 positive and T regulatory cells

Data table header descriptions
VALUE Quantile normalized intensities of samples exported from Expressionist

Data table
A_51_P100021 98.91243
A_51_P100034 3830.883
A_51_P100052 3.01962
A_51_P100063 3.638164
A_51_P100084 19.0353
A_51_P100099 95.61607
A_51_P100155 708.8774
A_51_P100174 181.9054
A_51_P100181 23.96131
A_51_P100218 1.286766
A_51_P100227 183.7305
A_51_P100238 1.39152
A_51_P100246 146.2959
A_51_P100289 1758.997
A_51_P100298 1.859078
A_51_P100309 1.640706
A_51_P100327 410.8429
A_51_P100347 37.96102
A_51_P100379 7.183138
A_51_P100428 1.792814

Total number of rows: 41174

Table truncated, full table size 878 Kbytes.

Supplementary file Size Download File type/resource
GSM798475_Treg_wildtype_3.txt.gz 7.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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