|
| Status |
Public on May 01, 2012 |
| Title |
TPC-1_construct_rep4 |
| Sample type |
RNA |
| |
|
| Source name |
TPC-1_PTCSC3_24h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: TPC-1
cell type: papillary thyroid carcinoma
transfected with: construct (PTCSC3-pcDNA3)
|
| Treatment protocol |
TPC-1 cell line cells were plated at 1x10^5 per 12-well dish and after 24 h transfected in quadruplicate with PTCSC3 expression vector and with empty vector (pcDNA3) as control using Lipofectamine2000 reagent (Invitrogen).
|
| Growth protocol |
TPC-1 cells were cultured in DMEM (Gibco) supplemented with 10% fetal calf serum (Gibco) and 1x MEM non-essential amino acids (Gibco) at 37C in 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
At 24 h after transfection the cells were washed once in PBS and total RNA was extracted (Trizol reagent, Invitrogen) and DNase-1 treated (Ambion).
|
| Label |
Cy3
|
| Label protocol |
RNA samples were labeled with cyanine-3 (Cy3) using the Agilent Low Input One–Color Labeling kit (Agilent Technologies, CA).
|
| |
|
| Hybridization protocol |
Samples were hybridized to the array for 17 hours at 65°C according to the manufacturer's protocol.
|
| Scan protocol |
Slides were scanned with an Agilent G2505C Microarray Scanner.
|
| Description |
24C_4
gene_expression_after_24h_from_transfection_expression_vector_(PTCSC3)_4
|
| Data processing |
Signal intensities were detected by Agilent DNA Microarray scanner and quantile normalized with R software using 'limma' bioconductor library.
|
| |
|
| Submission date |
Sep 17, 2011 |
| Last update date |
May 01, 2012 |
| Contact name |
Jaroslaw Piotr Jendrzejewski |
| E-mail(s) |
jaroslaw.jendrzejewski@osumc.edu
|
| Organization name |
The Ohio State University
|
| Department |
Comprehensive Cancer Center
|
| Lab |
Albert de la Chapelle
|
| Street address |
460W. 12th Avenue
|
| City |
Columbus |
| State/province |
Ohio |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platform ID |
GPL14550 |
| Series (1) |
| GSE32195 |
Analysis of impact of a novel RNA gene named Papillary Thyroid Carcinoma Susceptibility 3 (PTCSC3) on gene expression in papillary thyroid carcinoma cell line cells (TPC-1) |
|
