|
| Status |
Public on Apr 05, 2012 |
| Title |
Crohn's disease peripheral blood 2B |
| Sample type |
genomic |
| |
|
| Source name |
peripheral blood 2B
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
age (y): 43
tissue: Peripheral Blood
disease state: Crohn's disease
twin: Twin of sample 2A
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
For twin samples, genomic DNA was extracted and purified from peripheral blood samples using the Maxwell® 16 system from Promega with the Blood DNA purification kit. In order to increase the concentration of the extracted DNA, Milipore microcon filters were used. For other samples, either Gentra Puregene (Qiagen) for whole blood according to manufacturers recommendation, or the QuiAmp DNA mini kit (Qiagen) on isolated peripheral blood mononuclear cells (PBMCs) was used. BD Vacutainer® CPT™ Cell Preparation Tube with Sodium Citrate was used in the later case to isolate PBMCs from PBL.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
Bisulfite converted DNA was amplified, fragmented and hybridized to the Illumina Infinium HumanMethylation450 BeadChip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner settings
|
| Description |
Crohn's disease peripheral blood sample
|
| Data processing |
GenomeStudio software version 2010.3.0.30128
|
| |
|
| Submission date |
Sep 15, 2011 |
| Last update date |
Apr 05, 2012 |
| Contact name |
R. Alan Harris |
| E-mail(s) |
rharris1@bcm.edu
|
| Organization name |
Baylor College of Medicine
|
| Department |
Molecular and Human Genetics
|
| Street address |
1 Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE32148 |
DNA methylation in peripheral blood from individuals with Crohns' disease or ulcerative colitis and normal controls |
| GSE32149 |
DNA methylation in Crohns' disease and ulcerative colitis |
|
