|
| Status |
Public on Dec 11, 2023 |
| Title |
ans, rep 1 |
| Sample type |
SRA |
| |
|
| Source name |
Lateral non-spurred sepals
|
| Organism |
Delphinium anthriscifolium |
| Characteristics |
tissue: Lateral non-spurred sepals
genotype: ans
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the RNAprep Pure Plant Kit (TIANGEN)
Construction of an RNA sequencing library was uising Fast RNA-seq Lib Prep Kit V2
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Raw reads were filtered by removing adaptor sequences and low quality sequences to obtain high-quality reads
The clean reads were assembled into transcript using Trinity after the adaptors and the low quality reads were removed
The clean reads were separately mapped to the reference transcriptome by TopHat2
Fragments per kilobase per million mapped reads (FPKM) values were calculated using RSEM
Assembly: For de novo transcriptome analysis, RNA-seq reads were assembled into transcripts using the Trinity software.
Supplementary files format and content: Tab-delimited text file includes reads counts for each sample
|
| |
|
| Submission date |
Dec 11, 2023 |
| Last update date |
Dec 11, 2023 |
| Contact name |
Liping Guo |
| Organization name |
Northwest A&F University
|
| Street address |
Taicheng Road
|
| City |
Yangling |
| ZIP/Postal code |
712100 |
| Country |
China |
| |
|
| Platform ID |
GPL33999 |
| Series (1) |
| GSE249849 |
Asymmetric bending of lateral petals, a novel trait originated within Delphinium (Ranunculaceae), reinforces their mirrored helical rotation during flower opening |
|
| Relations |
| BioSample |
SAMN38759739 |
| SRA |
SRX22853698 |
