|
| Status |
Public on Dec 08, 2023 |
| Title |
Amor_P2_uPAR_negative |
| Sample type |
SRA |
| |
|
| Source name |
Pancreas
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Pancreas
genotype: WT
strain: C57BL6/J
treatment: Untreated
Sex: Male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Live uPAR positive or negative cells were sorted by uPAR staining (R&D, FAB531P) on a SONY SH800 cell sorter, stained with AO/PI (acridine orange/propidium iodide, Nexcelon ViaStain) and checked for viability >80% using a Countess FL II Automated cell counter.
10X Genomics NextGEM v3.1 Single Cell 3' Gene Expression libraries were prepared from sorted cells according to the manufacturer's instructions and sequenced on Illumina NextSeq2000 flow cells.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
10x Genomics
|
| Data processing |
Sequencing data was demultiplexed, mapped, and processed into gene/cell expression matrices using 10X Genomics' Cell Ranger software v7.1.0 (https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger). Gene expression reads were aligned to the mouse reference genome version gex-mm10-2020-A, available from the 10X Genomics website.
Assembly: mm10
Supplementary files format and content: Filtered single cell 3' cell/gene matrices in .h5 format generated by Cell Ranger for Liver + Adipose tissues
Supplementary files format and content: Filtered single cell 3' cell/gene matrices in .h5 format generated by Cell Ranger for Pancreas tissue
|
| |
|
| Submission date |
Sep 20, 2023 |
| Last update date |
Dec 08, 2023 |
| Contact name |
Yu-Jui Ho |
| E-mail(s) |
hoy@mskcc.org
|
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Department |
Cancer Biology & Genetics Program
|
| Street address |
417 E 68th St
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE243616 |
Prophilactic and long-lasting efficacy of senolytic CAR T cells against age-related metabolic dysfunction. |
|
| Relations |
| BioSample |
SAMN37475392 |
| SRA |
SRX21831071 |
