|
| Status |
Public on Nov 07, 2011 |
| Title |
HA ChIP in ES cells expressing HAZFP57 |
| Sample type |
SRA |
| |
|
| Source name |
HA ChIP in ES cells expressing HAZFP57
|
| Organism |
Mus musculus |
| Characteristics |
cell type: embryonic stem (ES) cells
chip antibody: HA
expressed transgene: HA tagged ZFP57
|
| Growth protocol |
Normal ES cell media
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Lysates were clarified from paraformaldehyde sonicated nuclei and protein-DNA complexes were isolated with antibody.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
Chromatin IP against HA
|
| Data processing |
Alignment: Sequence reads were obtained and mapped to the mouse genome (mm9) using the bowtie aligner. All reads mapping with three or fewer mismatches and reads not mapping to more than five locations in the genome were retained.
Peaks: Peak detection was performed with the ChIP-Seq Analysis Server (http://ccg.vital-it.ch/chipseq/)
|
| |
|
| Submission date |
Aug 03, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Simon Quenneville |
| E-mail(s) |
simon.quenneville@epfl.ch
|
| Organization name |
EPFL SV
|
| Street address |
station 15
|
| City |
Lausanne |
| ZIP/Postal code |
1003 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL9250 |
| Series (2) |
| GSE31181 |
ChIP-Seq of HA tagged ZFP57 and KAP1 in mouse ES cells |
| GSE31183 |
ZFP57/KAP1 recognize a methylated hexanucleotide to affect the chromatin and DNA methylation of imprinting control regions in embryonic stem cells |
|
| Relations |
| SRA |
SRX088969 |
| BioSample |
SAMN00691493 |
