|
| Status |
Public on Apr 24, 2024 |
| Title |
seminal, endometrial biopsy sample 6 |
| Sample type |
RNA |
| |
|
| Source name |
endometrium
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: endometrial biopsy
treatment: seminal
|
| Treatment protocol |
A Curette with Vacu-Lok syringe was used to collect the endometrial biopsy scratching the uterine lining. Cold saline solution was used to extract the endometrial biopsy from the tube. The samples were centrifuged (1000g, 5 min), the supernatant removed and the remaining endometrial sample was snap-frozen in liquid nitrogen and stored at −80°C until further analysis.
|
| Growth protocol |
not relevant
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using the miRNeasy Mini Kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
GeneChip Whole Transcript (WT) PLUS reagent kit was used to obtain the biotinylated cRNA (250 ng of RNA per sample used). All the procedures were performed following the manufacturer´s instructions.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized for 16 hr at 45C on Clariom D Array for human samples. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Genechip scanner GCS3000
|
| Description |
gene expression during implantation window
|
| Data processing |
Thermofisher Applied Biosystems Transcriptome Analysis Console version 4.0.14, SST-RMA normalization, limma t-test used for differential gene expression analysis
|
| |
|
| Submission date |
Aug 17, 2023 |
| Last update date |
Apr 24, 2024 |
| Contact name |
Laura Catalini |
| E-mail(s) |
laura.catalini@studenti.unicam.it
|
| Organization name |
Odense University Hospital
|
| Department |
Fertility clinic
|
| Street address |
Kløvervænget 23
|
| City |
Odense C |
| State/province |
Syddanmark |
| ZIP/Postal code |
DK-5000 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE241134 |
Endometrial transcriptomic changes after application of seminal plasma: a randomized controlled trial |
|
