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Sample GSM769572 Query DataSets for GSM769572
Status Public on Sep 30, 2011
Title Wild type 5W
Sample type RNA
Source name gastric mucosa, wild type, 5 weeks
Organism Mus musculus
Characteristics strain: C57BL/6J
genotype: wild type
age: 5 weeks
tissue: gastric mucosa
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the gastric mucosa stripped from the muscular layer of the glandular stomach using the RNeasy kit (Qiagen, Hilden, Germany).
Label Cy3
Label protocol Cy3-labeled cRNA was synthesized using the Quick Amp Labeling kit (Agilent Technologies).
Hybridization protocol 1.65 µg of Cy3-labeled cRNA were heat fragmented and applied to Agilent Whole Murine Genome Oligo ver. 2 (4 x 44K) arrays using a Gene Expression Hybridization kit (Agilent Technologies). Hybridization was carried out at 65ºC for 17 hours, and washing was performed using the Gene Expression Wash Pack (Agilent Technologies).
Scan protocol Slides were scanned with an Agilent Technologies Microarray Scanner, and the images were processed by Feature Extraction software (Agilent Technologies) with background correction to prepare the raw date output files.
Description W_5
Data processing Normalization was carried out using a percentile shift algorithm with parameter 75, and then data filtering was performed, using GeneSpring GX 11.0 software (Agilent Technologies).
Submission date Jul 31, 2011
Last update date Sep 30, 2011
Contact name Jun Nakayama
Organization name Shinshu University
Department Graduate School of Medicine
Lab Molecular Pathology
Street address Asahi 3-1-1
City Matsumoto
ZIP/Postal code 390-8621
Country Japan
Platform ID GPL11202
Series (1)
GSE31074 Essential role of gastric gland mucin in preventing gastric cancer in mice

Data table header descriptions
VALUE Normalized log2 signal intensity

Data table
A_55_P1989846 -6.304167
A_55_P1991598 -6.3022285
A_55_P2022211 -0.18604422
A_55_P1980764 -4.010335
A_55_P1964375 0.9586725
A_51_P128876 -0.72848415
A_55_P2121042 -6.290473
A_52_P219230 -6.287462
A_51_P207591 -1.9629464
A_55_P2131920 -6.281477
A_55_P2404223 -3.409504
A_55_P2101944 5.8302574
A_52_P358860 2.129077
A_51_P119031 -0.88311243
A_51_P309854 -6.3569064
A_51_P343900 1.4549685
A_51_P234359 -1.3283124
A_51_P487813 3.1628428
A_52_P613977 2.950615
A_55_P1957209 -6.235907

Total number of rows: 39483

Table truncated, full table size 928 Kbytes.

Supplementary file Size Download File type/resource
GSM769572.txt.gz 9.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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