|
| Status |
Public on Sep 30, 2011 |
| Title |
Wild type 5W |
| Sample type |
RNA |
| |
|
| Source name |
gastric mucosa, wild type, 5 weeks
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
genotype: wild type
age: 5 weeks
tissue: gastric mucosa
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the gastric mucosa stripped from the muscular layer of the glandular stomach using the RNeasy kit (Qiagen, Hilden, Germany).
|
| Label |
Cy3
|
| Label protocol |
Cy3-labeled cRNA was synthesized using the Quick Amp Labeling kit (Agilent Technologies).
|
| |
|
| Hybridization protocol |
1.65 µg of Cy3-labeled cRNA were heat fragmented and applied to Agilent Whole Murine Genome Oligo ver. 2 (4 x 44K) arrays using a Gene Expression Hybridization kit (Agilent Technologies). Hybridization was carried out at 65ºC for 17 hours, and washing was performed using the Gene Expression Wash Pack (Agilent Technologies).
|
| Scan protocol |
Slides were scanned with an Agilent Technologies Microarray Scanner, and the images were processed by Feature Extraction software (Agilent Technologies) with background correction to prepare the raw date output files.
|
| Description |
W_5
|
| Data processing |
Normalization was carried out using a percentile shift algorithm with parameter 75, and then data filtering was performed, using GeneSpring GX 11.0 software (Agilent Technologies).
|
| |
|
| Submission date |
Jul 31, 2011 |
| Last update date |
Sep 30, 2011 |
| Contact name |
Jun Nakayama |
| E-mail(s) |
jnaka@shinshu-u.ac.jp
|
| Organization name |
Shinshu University
|
| Department |
Graduate School of Medicine
|
| Lab |
Molecular Pathology
|
| Street address |
Asahi 3-1-1
|
| City |
Matsumoto |
| ZIP/Postal code |
390-8621 |
| Country |
Japan |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE31074 |
Essential role of gastric gland mucin in preventing gastric cancer in mice |
|
