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Sample GSM7668507

Query DataSets for GSM7668507

Status

Public on Nov 26, 2023

Title

Wt-FB2_326_251_S5

Sample type

SRA

Source name

fat body

Organism

Anopheles gambiae

Characteristics

tissue: fat body
genotype: mosGILT WT
treatment: Blood feeding

Treatment protocol

mosGILTnull and Wild type (WT,control) mosquitoes were fed on naïve mice

Extracted molecule

total RNA

Extraction protocol

The whole body, ovaries, and fat bodies were collected from blood-fed mosquitoes 24 h after completion of the meal. Total RNA was extracted from the whole body, ovaries, or fat bodies from mosquitoes. Trizol was added to the tissues and RNA was isolated using a RNeasy Mini Kit (QIAGEN)
RNA libraries were prepared for sequencing using standard Illumina protocols

Library strategy

RNA-Seq

Library source

transcriptomic

Library selection

cDNA

Instrument model

Illumina HiSeq 2500

Data processing

Partek Flow
RNAseq data were trimmed and aligned to the Anopheles genome, with associated annotation file using STAR (v2.7.3a).
The aligned reads were quantified to Ensembl transcripts using the Partek E/M algorithm and the subsequent steps were performed on gene-level annotation followed by total count normalization.
The counts generated were processed by gene set analysis (GSA).
Assembly: Anopheles gambiae PEST
Supplementary files format and content: tab-delimited text files include baseMean for each gene

Submission date

Jul 28, 2023

Last update date

Nov 26, 2023

Contact name

Xiaotian Tang

E-mail(s)

xiaotian.tang@yale.edu

Organization name

Yale School of Medicine

Department

Internal Medicine