|
| Status |
Public on Nov 22, 2011 |
| Title |
Estrogen (6 h) + Velcade J3 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7
treatment: estrogen (6h) + velcade stimulated
|
| Treatment protocol |
Where indicated, cells were pretreated with 50 nM Bortezomib for 15 minutes followed by 10 nM 17- -estradiol for 6 hours.
|
| Growth protocol |
Cells were grown in phenol-red free DMEM containing 5% charcoal-dextran treated FBS for 48 hours prior to treatment with Bortezomib or estrogen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiazol (Qiagen) according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Illumina TotalPrep RNA Amplification Kit (Ambion, Inc., Cat # IL1791); 200ng total RNA input
|
| |
|
| Hybridization protocol |
Illumina Whole-Genome Gene Expression Direct Hybridization Assay Guide (11286331)
|
| Scan protocol |
Illumina iScan Scanner
|
| Data processing |
log2 transformation of signal intensity, quantile normalization, R/Bioconductor, BeadStudio
|
| |
|
| Submission date |
Jul 25, 2011 |
| Last update date |
Nov 22, 2011 |
| Contact name |
Frank Kramer |
| E-mail(s) |
frank.kramer@med.uni-goettingen.de
|
| Organization name |
University Medical Center Göttingen
|
| Department |
Department of Medical Statistics
|
| Street address |
Humboldtallee 32
|
| City |
Goettingen |
| ZIP/Postal code |
37073 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE30931 |
Proteasome inhibition blocks estrogen-dependent gene transcription by decreasing histone H2B monoubiquitination in human breast cancer cells |
|
