|
| Status |
Public on Jul 22, 2011 |
| Title |
NZB Backcross animal 2424 |
| Sample type |
RNA |
| |
|
| Source name |
PyMT-induced mammary tumor
|
| Organism |
Mus musculus |
| Characteristics |
strain background: ((NZB x PyMT) x FVB) backcross
tissue: NZB x PyMT backcross mammary tumor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol (Invitrogen), following manufacturers protocol, RNeasy Mini Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
GeneChip® One-Cycle Target Labeling and Control Reagents (Affymetrix, P/N 900493) following manufacture’s protocol.
|
| |
|
| Hybridization protocol |
GeneChip® Hybridization, Wash and Stain Kit: (Affymetrix, P/N 900720) following manufacture’s protocol for One-Cycle cDNA Synthesis Kit Protocol for Eukaryotic Target Prepartion, Affymetrix)
|
| Scan protocol |
GCOSv1.4, Affymetrix Fluidics Station 450 (FS450_0001), Gene ChIP Scanner 3000 7G
|
| Description |
Spontaneous mammary tumors induced by MMTV-PyMT transgene
|
| Data processing |
GCOSv1.4, Scaling: default (500), Normalization: User defined=1, RMA normalization using BioConductor
|
| |
|
| Submission date |
Jul 21, 2011 |
| Last update date |
Jul 22, 2011 |
| Contact name |
Ying Hu |
| E-mail(s) |
yhu@mail.nih.gov, hunterk@mail.nih.gov
|
| Phone |
240 276 5273
|
| Organization name |
NIH
|
| Department |
NCI/CCR
|
| Lab |
CBIIT
|
| Street address |
9609 Medical Center Dr.
|
| City |
Rockville |
| State/province |
MD |
| ZIP/Postal code |
20850 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE30865 |
Gene expression of polyoma middle T antigen induced mammary tumors [NZB x PyMT] |
| GSE30866 |
Gene expression of polyoma middle T antigen induced mammary tumors |
|
