|
Status |
Public on Jul 22, 2011 |
Title |
AKXD subline 7 biological rep_1 [MOE430B] |
Sample type |
RNA |
|
|
Source name |
AKXD subline 7
|
Organism |
Mus musculus |
Characteristics |
strain background: AKXD x PyMT tissue: AKXD x PyMT mammary tumor
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol (Invitrogen), following manufacturers protocol, RNeasy Mini Kit (Qiagen)
|
Label |
biotin
|
Label protocol |
GeneChip® One-Cycle Target Labeling and Control Reagents (Affymetrix, P/N 900493) following manufacture’s protocol.
|
|
|
Hybridization protocol |
GeneChip® Hybridization, Wash and Stain Kit: (Affymetrix, P/N 900720) following manufacture’s protocol for One-Cycle cDNA Synthesis Kit Protocol for Eukaryotic Target Prepartion, Affymetrix)
|
Scan protocol |
GCOSv1.4, Affymetrix Fluidics Station 450 (FS450_0001), Gene ChIP Scanner 3000 7G
|
Description |
Spontaneous mammary tumors induced by MMTV-PyMT transgene AKXD2543sub7B
|
Data processing |
GCOSv1.4, Scaling: default (500), Normalization: User defined=1, RMA normalization using BioConductor
|
|
|
Submission date |
Jul 21, 2011 |
Last update date |
Jul 22, 2011 |
Contact name |
Ying Hu |
E-mail(s) |
yhu@mail.nih.gov, hunterk@mail.nih.gov
|
Phone |
240 276 5273
|
Organization name |
NIH
|
Department |
NCI/CCR
|
Lab |
CBIIT
|
Street address |
9609 Medical Center Dr.
|
City |
Rockville |
State/province |
MD |
ZIP/Postal code |
20850 |
Country |
USA |
|
|
Platform ID |
GPL340 |
Series (2) |
GSE30864 |
Gene expression of polyoma middle T antigen induced mammary tumors [AKXD x PyMT] |
GSE30866 |
Gene expression of polyoma middle T antigen induced mammary tumors |
|