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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 03, 2011 |
| Title |
ES cell feeder-free, w/ LIF 60 s CYH (100 ug/ml) ribo_mesc_yeslif Illumina HiSeq |
| Sample type |
SRA |
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| Source name |
ES cell
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| Organism |
Mus musculus |
| Characteristics |
treatment: ES cell feeder-free, w/ LIF 60 s CYH (100 ug/ml)
genetic background: 129/Ola
cell line: E14
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| Treatment protocol |
60 s cycloheximide (100 ug/ml)
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| Growth protocol |
feeder-free, with LIF
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| Extracted molecule |
total RNA |
| Extraction protocol |
Rnase I footprinting, ribosome pelleting, RNA extraction, preadenylated linker ligation, reverse transcription, cDNA circularization, PCR amplification
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina HiSeq 2000 |
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| Description |
Ribosome Profiling
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| Data processing |
Alignment to transcriptome (mm9) using 25nt seed and extension to linker sequence
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| Submission date |
Jul 21, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Nicholas T Ingolia |
| E-mail(s) |
ingolia@berkeley.edu, nick@ingolia.org
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| Phone |
510 664 7071
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| Organization name |
University of California, Berkeley
|
| Department |
Molecular and Cell Biology
|
| Lab |
Ingolia
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| Street address |
1 Barker Hall # 3202
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| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720 |
| Country |
USA |
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| Platform ID |
GPL13112 |
| Series (1) |
| GSE30839 |
Ribosome Profiling of Mouse Embryonic Stem Cells Reveals the Complexity of Mammalian Proteomes |
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| Relations |
| SRA |
SRX084825 |
| BioSample |
SAMN00672700 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM765302_ribo_mesc_yeslif_110414l7_trxs_norrna.align.txt.gz |
2.4 Gb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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