|
| Status |
Public on Nov 03, 2011 |
| Title |
ES cell feeder-free, w/ LIF 150 s HRT (1 ug/ml) then 60 s CYH (100 ug/ml) ribo_mesc_harr150s Illumina GAII |
| Sample type |
SRA |
| |
|
| Source name |
ES cell
|
| Organism |
Mus musculus |
| Characteristics |
treatment: ES cell feeder-free, w/ LIF 150 s HRT (1 ug/ml) then 60 s CYH (100 ug/ml)
genetic background: 129/Ola
cell line: E14
|
| Treatment protocol |
150 s harringtonine (1 ug/ml) followed by 60 s cycloheximide (100 ug/ml)
|
| Growth protocol |
feeder-free, with LIF
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rnase I footprinting, ribosome pelleting, RNA extraction, enzymatic polyadenylation, dT-primed reverse transcription, cDNA circularization, PCR amplification
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
Ribosome Profiling
|
| Data processing |
Alignment to transcriptome (mm9) using 25nt seed and extension to linker sequence
|
| |
|
| Submission date |
Jul 21, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Nicholas T Ingolia |
| E-mail(s) |
ingolia@berkeley.edu, nick@ingolia.org
|
| Phone |
510 664 7071
|
| Organization name |
University of California, Berkeley
|
| Department |
Molecular and Cell Biology
|
| Lab |
Ingolia
|
| Street address |
1 Barker Hall # 3202
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720 |
| Country |
USA |
| |
|
| Platform ID |
GPL9250 |
| Series (1) |
| GSE30839 |
Ribosome Profiling of Mouse Embryonic Stem Cells Reveals the Complexity of Mammalian Proteomes |
|
| Relations |
| SRA |
SRX084818 |
| BioSample |
SAMN00672693 |
