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Sample GSM76192 Query DataSets for GSM76192
Status Public on Jun 30, 2006
Title Dorsal_stg11.5_clutch4
Sample type RNA
Source name Dorsal half of stg11.5 gastrula
Organism Xenopus laevis
Characteristics Stage: 11.5;
Clutch: 4;
Tissue: Dorsal half of gastrula embryo;
Injection: eGFP
Treatment protocol Embryo was injected ventrally at the four-cell stage with eGFP mRNA. Embryos were sorted for proper ventral targeting at early stg. 10 by eGFP fluorescence. At stg. 11.5 embryos were bisected and 10 dorsal halves were saved for RNA isolation.
Growth protocol Embryos were grown in 1/3 MR.
Extracted molecule total RNA
Extraction protocol RNA isolation described in Hufton et al. 2005,
Label biotin
Label protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
Hybridization protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
Scan protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
Description The Ventral_stg11.5_clutch4 sample was derived from the same 10 eGFP injected embryos as this sample.
Data processing RMA method, values in the GEO database have not been log transformed.
Submission date Sep 26, 2005
Last update date Jun 30, 2006
Contact name Andrew Lee Hufton
Phone 650 736 0278
Organization name Stanford
Department Genetics
Lab Julie Baker
Street address 300 Pasteur Dr., Alway Bldg, Rm M313
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platform ID GPL1318
Series (1)
GSE3368 Genomic Analysis of the Xenopus Organizer

Data table header descriptions
VALUE RMA expression, not log'ed

Data table
AFFX-BioB-3_at 88.10347388
AFFX-BioB-5_at 114.4480443
AFFX-BioB-M_at 119.7502618
AFFX-BioC-3_at 249.512797
AFFX-BioC-5_at 268.3378954
AFFX-BioDn-3_at 1272.680881
AFFX-BioDn-5_at 432.996334
AFFX-CreX-3_at 2662.815951
AFFX-CreX-5_at 2818.746458
AFFX-DapX-3_at 9.834399755
AFFX-DapX-5_at 9.593997077
AFFX-DapX-M_at 13.11851943
AFFX-LysX-3_at 11.30041617
AFFX-LysX-5_at 7.588632868
AFFX-LysX-M_at 15.50823933
AFFX-PheX-3_at 57.37299791
AFFX-PheX-5_at 9.434990074
AFFX-PheX-M_at 11.02639283
AFFX-ThrX-3_at 37.43614564
AFFX-ThrX-5_at 16.69470852

Total number of rows: 15611

Table truncated, full table size 435 Kbytes.

Supplementary file Size Download File type/resource
GSM76192.CEL.gz 3.4 Mb (ftp)(http) CEL

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