|
| Status |
Public on Jun 30, 2006 |
| Title |
Dkk-1_stg11.5_clutch4 |
| Sample type |
RNA |
| |
|
| Source name |
Ventral half of stg11.5 gastrula, injected with dkk-1
|
| Organism |
Xenopus laevis |
| Characteristics |
Stage: 11.5;
Clutch: 4;
Tissue: Ventral half of gastrula embryo;
Injection: dkk-1 and eGFP
|
| Treatment protocol |
Embryo was injected ventrally at the four-cell stage with dkk-1 plasmid and eGFP mRNA. Embryos were sorted for proper ventral targeting at early stg. 10 by eGFP fluorescence. At stg. 11.5 embryos were bisected and 10 ventral halves were saved for RNA isolation.
|
| Growth protocol |
Embryos were grown in 1/3 MR.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation described in Hufton et al. 2005,
|
| Label |
biotin
|
| Label protocol |
Manufacturer's protocols (Affymetrix, Santa Clara, CA).
|
| |
|
| Hybridization protocol |
Manufacturer's protocols (Affymetrix, Santa Clara, CA).
|
| Scan protocol |
Manufacturer's protocols (Affymetrix, Santa Clara, CA).
|
| Description |
N/A
|
| Data processing |
RMA method, values in the GEO database have not been log transformed.
|
| |
|
| Submission date |
Sep 26, 2005 |
| Last update date |
Jun 30, 2006 |
| Contact name |
Andrew Lee Hufton |
| E-mail(s) |
alhufton@stanford.edu
|
| Phone |
650 736 0278
|
| URL |
http://www.stanford.edu/group/bakerlab/
|
| Organization name |
Stanford
|
| Department |
Genetics
|
| Lab |
Julie Baker
|
| Street address |
300 Pasteur Dr., Alway Bldg, Rm M313
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL1318 |
| Series (1) |
| GSE3368 |
Genomic Analysis of the Xenopus Organizer |
|
