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Sample GSM76191 Query DataSets for GSM76191
Status Public on Jun 30, 2006
Title Dkk-1_stg11.5_clutch4
Sample type RNA
Source name Ventral half of stg11.5 gastrula, injected with dkk-1
Organism Xenopus laevis
Characteristics Stage: 11.5;
Clutch: 4;
Tissue: Ventral half of gastrula embryo;
Injection: dkk-1 and eGFP
Treatment protocol Embryo was injected ventrally at the four-cell stage with dkk-1 plasmid and eGFP mRNA. Embryos were sorted for proper ventral targeting at early stg. 10 by eGFP fluorescence. At stg. 11.5 embryos were bisected and 10 ventral halves were saved for RNA isolation.
Growth protocol Embryos were grown in 1/3 MR.
Extracted molecule total RNA
Extraction protocol RNA isolation described in Hufton et al. 2005,
Label biotin
Label protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
Hybridization protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
Scan protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
Description N/A
Data processing RMA method, values in the GEO database have not been log transformed.
Submission date Sep 26, 2005
Last update date Jun 30, 2006
Contact name Andrew Lee Hufton
Phone 650 736 0278
Organization name Stanford
Department Genetics
Lab Julie Baker
Street address 300 Pasteur Dr., Alway Bldg, Rm M313
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platform ID GPL1318
Series (1)
GSE3368 Genomic Analysis of the Xenopus Organizer

Data table header descriptions
VALUE RMA expression, not log'ed

Data table
AFFX-BioB-3_at 108.5161531
AFFX-BioB-5_at 125.8197857
AFFX-BioB-M_at 125.8871311
AFFX-BioC-3_at 331.149989
AFFX-BioC-5_at 321.3422793
AFFX-BioDn-3_at 1417.832117
AFFX-BioDn-5_at 559.702352
AFFX-CreX-3_at 3406.092737
AFFX-CreX-5_at 2948.206781
AFFX-DapX-3_at 9.831598496
AFFX-DapX-5_at 10.00492883
AFFX-DapX-M_at 13.16730327
AFFX-LysX-3_at 13.19428255
AFFX-LysX-5_at 8.316410663
AFFX-LysX-M_at 16.1699465
AFFX-PheX-3_at 63.4184187
AFFX-PheX-5_at 10.8116964
AFFX-PheX-M_at 10.93617251
AFFX-ThrX-3_at 38.0861882
AFFX-ThrX-5_at 18.81329966

Total number of rows: 15611

Table truncated, full table size 435 Kbytes.

Supplementary file Size Download File type/resource
GSM76191.CEL.gz 3.4 Mb (ftp)(http) CEL

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