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Sample GSM76164 Query DataSets for GSM76164
Status Public on Jun 30, 2006
Title Noggin_stg10_clutch2
Sample type RNA
Source name Ventral half of stg10 gastrula, injected with noggin
Organism Xenopus laevis
Characteristics Stage: 10;
Clutch: 2;
Tissue: Ventral half of gastrula embryo;
Injection: Noggin and eGFP
Treatment protocol Embryo was injected ventrally at the four-cell stage with noggin plasmid and eGFP mRNA. Embryos were sorted for proper ventral targeting at early stg. 10 by eGFP fluorescence. At stg. 10 embryos were bisected and 10 ventral halves were saved for RNA isolation.
Growth protocol Embryos were grown 1/3 MR.
Extracted molecule total RNA
Extraction protocol RNA isolation described in Hufton et al. 2005,
Label biotin
Label protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
Hybridization protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
Scan protocol Manufacturer's protocols (Affymetrix, Santa Clara, CA).
Description N/A
Data processing RMA method, values in the GEO database have not been log transformed.
Submission date Sep 26, 2005
Last update date Jun 30, 2006
Contact name Andrew Lee Hufton
Phone 650 736 0278
Organization name Stanford
Department Genetics
Lab Julie Baker
Street address 300 Pasteur Dr., Alway Bldg, Rm M313
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platform ID GPL1318
Series (1)
GSE3368 Genomic Analysis of the Xenopus Organizer

Data table header descriptions
VALUE RMA expression, not log'ed

Data table
AFFX-BioB-3_at 147.8919791
AFFX-BioB-5_at 193.4854137
AFFX-BioB-M_at 232.0826719
AFFX-BioC-3_at 285.3648835
AFFX-BioC-5_at 349.3244952
AFFX-BioDn-3_at 1536.062284
AFFX-BioDn-5_at 265.7424696
AFFX-CreX-3_at 4023.624616
AFFX-CreX-5_at 3332.841515
AFFX-DapX-3_at 9.549339834
AFFX-DapX-5_at 9.291219712
AFFX-DapX-M_at 13.0906334
AFFX-LysX-3_at 10.99748707
AFFX-LysX-5_at 8.287373823
AFFX-LysX-M_at 16.23618749
AFFX-PheX-3_at 70.32681617
AFFX-PheX-5_at 10.73220788
AFFX-PheX-M_at 11.26134312
AFFX-ThrX-3_at 33.17510209
AFFX-ThrX-5_at 15.36728079

Total number of rows: 15611

Table truncated, full table size 435 Kbytes.

Supplementary file Size Download File type/resource
GSM76164.CEL.gz 3.3 Mb (ftp)(http) CEL

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