|
| Status |
Public on Aug 26, 2011 |
| Title |
myometrium, patient MY53, MED12wt |
| Sample type |
RNA |
| |
|
| Source name |
myometrium, patient MY53, MED12wt
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: myometrium
med12: wt
patient: MY53
|
| Treatment protocol |
-
|
| Growth protocol |
-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using TRIzol® Reagent and purified with RNeasy® MinElute™ clean up kit according to manufacturers’ instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 10ug of total RNA
|
| |
|
| Hybridization protocol |
Fragmented cRNA was hybridized according to the manufacturer´s intructions
|
| Scan protocol |
According to the standard Affymetrix procedures
|
| Description |
Myometrium gene expression data from patient MY53, MED12wt
|
| Data processing |
Gene expression data was processed and analyzed with Partek Genomic Suite™ v. 6.5 (Partek Incorporated, St. Louis, MO, USA) using Brainarray Custom CDF files (HGU133Plus2_Hs_ENSG,v.14.1.0, http://brainarray.mbni.med.umich.edu/ Brainarray/default.asp).
|
| |
|
| Submission date |
Jul 14, 2011 |
| Last update date |
Aug 27, 2011 |
| Contact name |
Miika Mehine |
| E-mail(s) |
miika.mehine@helsinki.fi
|
| Organization name |
University of Helsinki
|
| Street address |
P.O. Box 63 (Haartmaninkatu 8)
|
| City |
Helsinki |
| ZIP/Postal code |
FI-00014 |
| Country |
Finland |
| |
|
| Platform ID |
GPL13916 |
| Series (1) |
| GSE30673 |
Comparison of Gene expression profiles between myometrium, MED12 mutation positive uterine leiomyomas and MED12 wildtype uterine leiomyomas |
|
