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Sample GSM75880 Query DataSets for GSM75880
Status Public on May 01, 2006
Title coronary smooth muscle cells metoprolol replicate 2
Sample type RNA
Source name coronary smooth muscle cells (hcaSMC), treated with metoprolol for 72 hours
Organism Homo sapiens
Characteristics metoprolol treated muscle cells
Extracted molecule total RNA
Extraction protocol total RNA of human coronary artery smooth muscle (hcaSMC) cultivated in SMGM-2-MV bulletkit medium were isolated after incubation with metoprolol for 72 hours using Trizol and purified with RNeasy spin columns.
Label biotin
Label protocol Approximately 7 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description human coronary artery smooth muscle Cells, treated with metoprolol, replicate 2
Data processing Affymetrix Microarray Suite version 5.0
Submission date Sep 23, 2005
Last update date Sep 23, 2005
Contact name Bernhard R. Brehm
Phone +4970712982711
Fax +497071360245
Organization name University of Tuebingen
Street address Otfried-Mueller-Str. 10
City Tuebingen
ZIP/Postal code 72076
Country Germany
Platform ID GPL96
Series (1)
GSE3356 Effects of metoprolol and nebivolol on gene expresion in human coronary smooth muscle cells

Data table header descriptions
VALUE 'signal' a measure of the abundance of a transcript
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 95.7 P 0.02002
AFFX-BioB-M_at 148.8 P 0.00015
AFFX-BioB-3_at 52.8 P 0.01828
AFFX-BioC-5_at 229.6 P 0.00045
AFFX-BioC-3_at 148.1 P 0.0002
AFFX-BioDn-5_at 190.7 P 0.00017
AFFX-BioDn-3_at 1698.2 P 0.00034
AFFX-CreX-5_at 2230.1 P 0.00004
AFFX-CreX-3_at 3642.4 P 0.00004
AFFX-DapX-5_at 11.9 A 0.22764
AFFX-DapX-M_at 18.4 A 0.27515
AFFX-DapX-3_at 1.4 A 0.95352
AFFX-LysX-5_at 1.3 A 0.83414
AFFX-LysX-M_at 5.7 A 0.68628
AFFX-LysX-3_at 2.3 A 0.57404
AFFX-PheX-5_at 1 A 0.96902
AFFX-PheX-M_at 1.7 A 0.91641
AFFX-PheX-3_at 6.1 A 0.72485
AFFX-ThrX-5_at 3 A 0.81487
AFFX-ThrX-M_at 3.4 A 0.64555

Total number of rows: 22283

Table truncated, full table size 562 Kbytes.

Supplementary file Size Download File type/resource
GSM75880.CEL.gz 3.5 Mb (ftp)(http) CEL
GSM75880.EXP.gz 565 b (ftp)(http) EXP

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