|
| Status |
Public on Jul 09, 2011 |
| Title |
DMSO Control after 24hr, replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Cell culture of Melanoma Cell Line - A375
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Melanoma Cell Line - A375
treatment: DMSO Control
time: 24hr
|
| Treatment protocol |
0.065% DMSO or 650nM MLN4924 were added to 2 million A375 cells and incubated for 1, 2, 4, 8, or 24 hours before recovery for RNA isolation and Gene Expression.
|
| Growth protocol |
A375 cells were maintained at 37oC in DMEM medium supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine and 100 U/ml penicillin/100 g/ml streptomycin (Invitrogen).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were precipitated, PBS washed, lysed using Qiagen RLT Buffer, and then RNA was extracted with Ambion MegaMax kit using King Fisher automated platform. All protocoles were executed following manufacturers recommendations.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using modified MesageAmp-based protocols (Ambion Inc) from 1.5ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133Plus 2.0 Array in an Affymetrix Model 640 hybridization oven . GeneChips were washed and stained on an Affymetrix FS450 station.
|
| Scan protocol |
The arrays were scanned on an Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from treated cells
|
| Data processing |
The data were analyzed with Expression Console software from Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
|
| |
|
| Submission date |
Jul 08, 2011 |
| Last update date |
Apr 11, 2014 |
| Contact name |
Hugues Bernard |
| E-mail(s) |
bernard@mpi.com
|
| Organization name |
Millennium: The Takeda Oncology company
|
| Department |
Molecular Pathology
|
| Street address |
35 Landsdowne St.
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE30531 |
Expression data of A375 melanoma cells after DMSO or MLN4924 treatment from 1 hour to 24 hour |
|
