|
| Status |
Public on Oct 25, 2012 |
| Title |
R4 |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA from human prostate cancer, Gleason 3+4
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: prostate
gleason score: 3+4
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ultraspec extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
100ng of total RNA from each sample, isolated from prostate biopsies included in OCT, was processed, labelled according to the Affymetrix GeneChip® Whole Transcript Sense Target Labelling Assay
|
| |
|
| Hybridization protocol |
Sample was hybridized to Affymetrix Human Exon 1.0 ST array using a Affymetrix GeneChip Hybridization Oven 645
|
| Scan protocol |
GeneChip was scanned using Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Gene expression data from Human prostate cancer
|
| Data processing |
The data were analyzed using the RMA algorithm and then LIMMA was applied to calculate significant differential expression between samples and using core exons. The genome build was hg18.
|
| |
|
| Submission date |
Jul 08, 2011 |
| Last update date |
Oct 25, 2012 |
| Contact name |
Laia Agell |
| E-mail(s) |
lagell@imim.es
|
| Phone |
+34932483423
|
| Fax |
+34932483031
|
| Organization name |
FIMIM
|
| Department |
Pathology
|
| Street address |
Passeig Maritim 25-29
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL9793 |
| Series (1) |
| GSE30521 |
Expression data from prostate cancer samples |
|
