|
| Status |
Public on Jun 30, 2024 |
| Title |
EXP1-rep2-GRP158-72 H |
| Sample type |
RNA |
| |
|
| Source name |
human trabecular meshwork (HTM) cells.
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HTM cells transfected with TIGR/MYOC cDNA (TM-1).
cell type: human trabecular meshwork (HTM) cells
treatment: GRP158-72 H
|
| Treatment protocol |
Exp1: cells were treated with doxycycline at 100 ng/mL for 72 hours to induce GRP128. Exp2A: cells were treated with doxycycline at 500 ng/mL for 24 hours. Exp2B: cells were treated with doxycycline at 500 ng/mL for 96 hours.
Cells transfected with only lenti-vector were used as a negative control.
|
| Growth protocol |
TM-1 cells were seeded at 10'5/mL in the wells of 6-well plates. Cells were maintained in reduced serum (2.5%)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from each culture plate well using Aurum (Bio-Rad, Hercules, CA)
|
| Label |
Cy3
|
| Label protocol |
cRNA was labelled with Cyanine-3
|
| |
|
| Hybridization protocol |
Cyanine-3-labelled cRNA was hybridized on Agilent SurePrint G3 Human GE 8×60K Microarray v2.
|
| Scan protocol |
Arrays were scanned with an Agilent DNA Microarray Scanner at a 3-um scan resolution
|
| Description |
replicate 2
|
| Data processing |
Image data were processed with Agilent Feature Extraction 11.0.1.1, and processed signals were quantile normalized with Agilent GeneSpring 12.0.
The normalized expression intensities for all probes were subjected to a two-way analysis of variance analysis (ANOVA). Significantly differentially expressed genes were selected using criteria p-value (or FDR using the Benjimini-Hochberg method) < 0.05 and fold-change > 1.2.
|
| |
|
| Submission date |
Jun 28, 2023 |
| Last update date |
Jun 30, 2024 |
| Contact name |
Jose Echenique |
| E-mail(s) |
jechenique@unc.edu.ar
|
| Phone |
03515517034
|
| Organization name |
School of Chemistry Sciences, National University of Cordoba
|
| Department |
Clinical Biochemistry
|
| Lab |
Molecular Microbiology
|
| Street address |
Edificio Integrador, Medina Allende S/N, Ciudad Universitaria
|
| City |
Córdoba |
| State/province |
Córdoba |
| ZIP/Postal code |
5000 |
| Country |
Argentina |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE236016 |
GPR158 Activates Cellular Stress Responses in Trabecular Meshwork Cells of the Eye’s Aqueous Outflow Pathways: Implications for Ocular Hypertension |
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