|
| Status |
Public on Jul 24, 2023 |
| Title |
Adipocyte + SKOV3ip1 co-culture [EL6] |
| Sample type |
RNA |
| |
|
| Source name |
Adipose tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Benign Tissue
|
| Treatment protocol |
Cancer cells were co-cultured with primary human omental adipocytes at 1:3 (packed cell volume)
|
| Growth protocol |
Cells were grown in DMEM:F12 media supplemented with 0.1% fatty acids-free bovine serum albumin, 100 U/ml penicillin, and 100 µg/ml streptomycin .
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using RNeasy kit after in-column DNase digestion.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was generated using Illumina Total Prep RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Direct Hybridization Assay
|
| Scan protocol |
Arrays were scanned on an Illumina’s Bead Station 500GX Genetic Analysis System
|
| Description |
Replicate 1
EL6
|
| Data processing |
The raw data were processed by Illumina Genome Studio v2011.1 and Partek Genomics suite v6.6.
|
| |
|
| Submission date |
Jun 23, 2023 |
| Last update date |
Jul 24, 2023 |
| Contact name |
Abir Mukherjee |
| E-mail(s) |
abirm@uchicago.edu
|
| Organization name |
university of Chicago
|
| Street address |
929 E 57th street, Gordon center for integrative sciences, room w107K
|
| City |
Chicago |
| State/province |
Illinois |
| ZIP/Postal code |
60615 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE235641 |
Bi-directional interactions of omental adipocytes and SKOV3ip1 cells |
|
