|
| Status |
Public on Dec 21, 2012 |
| Title |
WT-HighFatDiet-rep4 |
| Sample type |
RNA |
| |
|
| Source name |
White adipose tissue dissected from wild type mice on a high fat diet for 8 weeks
|
| Organism |
Mus musculus |
| Characteristics |
background strain: C57BL6/J
cre transgene: None
diet: High Fat
|
| Treatment protocol |
White adipose tissue dissected from mice andimmediately processed immediately to isolate RNA
|
| Growth protocol |
12 week old mice with indicated genotypes fed high or low fat diets for 8 weeks-feeding started at 4 weeks of age
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was DNAseI treated and further purified using the QIAgen Rneasy mini columns
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA according to the Affymetrix Expression Analysis Technical Manual.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
Gene expression data from mouse white adipose tissue with Sirt1 fed a high fat diet
|
| Data processing |
R version 2.11.1 (x86_64-pc-linux-gnu), affyPLM_1.24.1, preprocessCore_1.10.0, gcrma_2.20.0, affy_1.26.1, Biobase_2.8.0
|
| |
|
| Submission date |
Jun 27, 2011 |
| Last update date |
Dec 21, 2012 |
| Contact name |
Charles Arthur Whittaker |
| E-mail(s) |
charliew@mit.edu
|
| Organization name |
Koch Institute
|
| Street address |
77 Mass Ave 76-189
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02152 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE30247 |
High Fat Diet Triggers SIRT1 Cleavage in Adipose Tissue Providing a Link between Dietary Stress and Metabolic Dysfunction. |
|
