|
| Status |
Public on Jan 17, 2024 |
| Title |
R-2A ROR2 KD day14, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Human induced pluripotent stem cell
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HiPS-RIKEN-2A
cell type: Human induced pluripotent stem cell
treatment: shROR2
treatment: hiPSC-derived neural progenitor cell day14
|
| Treatment protocol |
HiPSCs were differentiated into NPCs by using the STEMdiff SMADi Neural Induction Kit (Stem Cell Technologies), according to the manufacturer instructions. The cells were passaged every 7 days until day 21 post-differentiation.
|
| Growth protocol |
Undifferentiated hiPSCs were maintained on iMatrix-511 in StemFit AK02 medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the hiPSC-derived NPC cells using RNeasy Mini Kit (QIAGEN), according to the manufacturer instructions.
|
| Label |
Biotin
|
| Label protocol |
Labeling extracted total RNA was performed using the Gene ChipTM WT PLUS Reagent Kit (Thermo Fisher Scientific) according to manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Hybridization was performed in a Gene Chip Hybridization Oven 645 for 16 h at 45 °C.
|
| Scan protocol |
Gene chips were scanned using the GeneChip Scanner 3000.
|
| Data processing |
Array-Quality Control was done using the Transcriptome Analysis Console (TAC) Software (version 4.0.3.14). Gene expression intensities were normalized and summarized with SST robust multiarray average algorithm (Affymetrix): SST-RMA.
|
| |
|
| Submission date |
May 23, 2023 |
| Last update date |
Jan 17, 2024 |
| Contact name |
Takuya Kuroda |
| E-mail(s) |
kuroda@nihs.go.jp
|
| Organization name |
National Institute of Health Sciences
|
| Street address |
3-25-26 Tonomachi, Kawasaki Ward,
|
| City |
Kawasaki City |
| State/province |
Kanagawa |
| ZIP/Postal code |
210-9501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23126 |
| Series (1) |
| GSE233228 |
Gene expression profilings of neural progenitor cell derived from hiPSC with ROR2 knockdown |
|
