|
| Status |
Public on Jul 19, 2011 |
| Title |
Cervical tumor biopsy, P-288 |
| Sample type |
RNA |
| |
|
| Source name |
Cervical tumor
|
| Organism |
Homo sapiens |
| Characteristics |
cohort (gse36562 study): Validation cohort
hypoxia score: Low
tissue: cervical tumor
figo stage: 2B
histology: Squamous cell carcinoma
cohort: basic cohort
lymph node status (gse38433 study): 0
cohort (gse38964 study): Validation cohort
3p status: Not determined
|
| Treatment protocol |
The samples were immediately snap-frozen in liquid nitrogen and stored at -80 C until used for analysis. Biopsies with more than 50% tumor cells in HE stained sections from the central part of the specimen were used further.
|
| Growth protocol |
1-4 tumor samples wer collected from each patient at the time of diagnosis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by double precipitation with isopropanol and final precipitation with 5M lithium chloride. RNA from different biopsies of the same tumor was pooled. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Basic cohort
P-288
|
| Data processing |
The data were normalised using quantile normalisation in BeadStudio 3.1.3.0
|
| |
|
| Submission date |
Jun 08, 2011 |
| Last update date |
Apr 28, 2015 |
| Contact name |
Heidi Lyng |
| E-mail(s) |
heidi.lyng@rr-research.no
|
| Phone |
4722781478
|
| Organization name |
Oslo University Hospital
|
| Department |
Department of Radiation Biology
|
| Street address |
Montebello
|
| City |
Oslo |
| ZIP/Postal code |
0310 |
| Country |
Norway |
| |
|
| Platform ID |
GPL6884 |
| Series (5)
|
| GSE29817 |
Membranous Expression of Ectodomain Isoforms of the Epidermal Growth Factor Receptor (EGFR) Predicts Outcome after Chemoradiotherapy of Lymph Node Negative Cervical Cancer |
| GSE36562 |
Hypoxia-induced gene expression in chemoradioresistant cervical cancer revealed by dynamic contrast enhanced MRI |
| GSE38964 |
Identification of eight candidate target genes of the prognsotic 3p loss in cervical cancer by integrative genomic profiling |
| GSE72723 |
Integrative analysis of DCE-MRI and gene expression profiles in construction of a gene classifier for assessment of hypoxia-related risk of chemoradiotherapy failure in cervical cancer |
| GSE75034 |
Selection of reference genes for gene expression studies related to hypoxia in cervical cancer |
|
| Relations |
| Affiliated with |
GSE68339 |
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