|
| Status |
Public on Dec 01, 2011 |
| Title |
Embryo-injected-EXP1-rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Nkx2-5/GFP injected_EXP1
|
| Organism |
Xenopus laevis |
| Characteristics |
development stage: St. 11.5
tissue: whole embryo
sample origin/type: single embryo
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was collected by homogenization in Invitrogen TRIzol reagent and further purified using an Qiagen RNeasy micro kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard 2-cycle Affymetrix protocol from 100ng of total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 17 hr at 45C on Xenopus Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using the standard Affymetrix protocol.
|
| Description |
Gene expression data from whole embryo
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
|
| |
|
| Submission date |
Jun 07, 2011 |
| Last update date |
May 10, 2017 |
| Contact name |
Marcus Breese |
| Organization name |
UCSF
|
| Street address |
1550 Fourth St, 382
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL1318 |
| Series (2) |
| GSE29791 |
Identification of putative targets of Nkx2-5 in Xenopus laevis using gene expression analysis and cross-species annotation |
| GSE29816 |
Identification of putative targets of Nkx2-5 in Xenopus laevis using gene expression analysis and cross-species. |
|
