|
| Status |
Public on Aug 01, 2011 |
| Title |
SG200 biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
U. maydis strain SG200 grown for 48 h in Charcoal minimal media
|
| Organism |
Mycosarcoma maydis |
| Characteristics |
strain: SG200 is a FB1 derivative with the insertion of a compatible b-mating locus
genetic background: SG200
genotype: wild type
|
| Growth protocol |
SG200 and SG200deltatup1 were grown to an OD600 of 0.5 at 28°C in YEPSL (0.4% peptone, 1% yeast extract, 0.4% sucrose). Then, cells were washed twice with sterile water and spread out on charcoal array plates (6.25 % (w/v) Holiday salt solution, 1% vitamin solution, 30 mM L-glutamine, 1 % (w/v) glucose, pH 7.0 (filter-sterilized) and mixed with 2% agar, 1% charcoal sterile solution) at a concentration of OD600 of 0.1 per cm2 and grown for 48 hours at 22ºC.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the TRIZOL-reagent (Invitrogen) according to the manufacturer’s instructions. RNA samples were further column purified (Qiagen RNeasy) and the quality checked using a Bioanalyzer with a RNA 6000 Nano LabChip kit (Agilent).
|
| Label |
biotin/phycoerythrin
|
| Label protocol |
Target preparation was performed according to the standard Affymetrix protocol using the Enzo BioArray RNA transcript labeling kit, except for a reaction temperature of 50°C for first-strand cDNA synthesis.
|
| |
|
| Hybridization protocol |
Affymetrix EukGE-WS2 on a GeneChip Fluidics Station 400
|
| Scan protocol |
Affymetrix GCS2500
|
| Data processing |
MAS5.0
|
| |
|
| Submission date |
May 27, 2011 |
| Last update date |
Aug 01, 2011 |
| Contact name |
Jose I Ibeas |
| E-mail(s) |
joibecor@upo.es
|
| Phone |
34 954 349379
|
| Fax |
34 954 349376
|
| Organization name |
Universidad Pablo de Olavide
|
| Department |
Biolgía Molecular e Ingeniería Bioquímica
|
| Lab |
Genética
|
| Street address |
Carretera de Utrera Km 1
|
| City |
Sevilla |
| ZIP/Postal code |
41013 |
| Country |
Spain |
| |
|
| Platform ID |
GPL3681 |
| Series (1) |
|
