|
| Status |
Public on Jan 25, 2024 |
| Title |
Ctrl_1_S1_R1_001 |
| Sample type |
SRA |
| |
|
| Source name |
THD' neurons
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell type: THD' neurons
genotype: WT
time: 72 hrs APF
|
| Growth protocol |
Drosophila strains were grown on standard cornmeal medium supplemented with yeast (ND) at 25°C under a light-dark cycle of 12h and 12h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from at least 600 THD’ neurons from the relevant genotypes was subjected to 14 cycles of PCR amplification (SMARTer Seq V4 Ultra Low Input RNA Kit; Takara Bio). 1 ng of amplified RNA was used to prepare cDNA libraries (Nextera XT DNA library preparation kit; Illumina)
RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Reads were aligned to the genome using HISAT2 (Kim et al., 2015, 2019)
Mapped sequence reads were aligned to the genome to obtain read counts using Featurecounts (Liao et al., 2014)
Differential expression analysis was performed using three independent methods: DESeq2, limma-voom, and edgeR
Assembly: dm6
Supplementary files format and content: tab-delimited text files include read counts for each Sample
|
| |
|
| Submission date |
Apr 20, 2023 |
| Last update date |
Jan 25, 2024 |
| Contact name |
Gaiti Hasan |
| E-mail(s) |
gaiti@ncbs.res.in
|
| Organization name |
National Centre for Biological Sciences
|
| Lab |
Gaiti Hasan Lab
|
| Street address |
GKVK Campus, Bellary Road
|
| City |
Bangalore |
| State/province |
Karnataka |
| ZIP/Postal code |
560065 |
| Country |
India |
| |
|
| Platform ID |
GPL17275 |
| Series (1) |
| GSE230134 |
Orai-mediated calcium entry regulates the excitability of central dopaminergic neurons |
|
| Relations |
| BioSample |
SAMN34264543 |
| SRA |
SRX20022008 |
