|
| Status |
Public on Oct 28, 2011 |
| Title |
Whole blood LTBI_251485039469_1_1 |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral blood LTBI
|
| Organism |
Homo sapiens |
| Characteristics |
group: healthy donors latently infected with M. tuberculosis
gender: Female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from 2,5 ml of peripheral whole blood collected in PAXgene tubes, using the PAXgene Blood RNA Kit (PreAnalystics, Europe BD, Belgium).
|
| Label |
Cy3
|
| Label protocol |
Total RNA was labeled with the Fluorescent Linear Amplification Kit (Agilent Technologies) following manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Quantity and labelling efficiency were verified before hybridization of the samples to whole-genome oligonucleotide microarray (Agilent Technologies).
|
| Scan protocol |
Scanned on an Agilent Scanner G2505B. Image analysis was performed with Feature Extraction software (version 6.1.1, Agilent Technologies).
|
| Description |
LTBI whole blood RNA
|
| Data processing |
Agilent Feature Extraction Software (v 6.1.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Apr 14, 2011 |
| Last update date |
Oct 28, 2011 |
| Contact name |
Jeroen Maertzdorf |
| Organization name |
MPIIB
|
| Street address |
Chariteplatz 1
|
| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE28623 |
Pathway and functional association analysis of whole blood gene expression profiles reveal functional networks underlying TB pathogenesis. [Agilent-014850] |
|
