|
| Status |
Public on May 22, 2023 |
| Title |
SEQTR_vs_RACE_Replicate3_bis |
| Sample type |
SRA |
| |
|
| Source name |
peripheral blood mononuclear cell (PBMC) donnoe 4
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: PBMC
repertoire: beta TCR repertoire
|
| Growth protocol |
PBMC were obtained from leukapheresis of healthy donor.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mRNA were extracted using the Dynabeads mRNA DIRECT purification kit
Custum protocol for TCR sequencing describe in the manuscript.
ImmunoSEQ commercially available kit for TCR amplification
SMARTer (Takara) commercially available kit for TCR amplification
|
| |
|
| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
NextSeq 1000 |
| |
|
| Data processing |
Ad hoc perl script
MiXCR software from MiLaboratories
Assembly: TCR alignment was performed using the IMGT database
Supplementary files format and content: Text file with the list of TCRs
Library strategy: Targeted amplification
|
| |
|
| Submission date |
Feb 23, 2023 |
| Last update date |
May 23, 2023 |
| Contact name |
Raphael Genolet |
| E-mail(s) |
raphael.genolet@chuv.ch
|
| Organization name |
Centre Hospitalier Universitaire Vaudois (CHUV)
|
| Department |
Departement d'oncologie UNIL-CHUV
|
| Street address |
Biopole 3, Chemin des Boveresses 155
|
| City |
Epalinges |
| ZIP/Postal code |
1066 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL30882 |
| Series (1) |
| GSE225984 |
Novel TCR sequencing and cloning methods for sensitive and quantitative interrogation of repertoires and rapid isolation of tumor-reactive TCRs |
|
| Relations |
| BioSample |
SAMN33426755 |
| SRA |
SRX19488287 |
