|
| Status |
Public on Apr 08, 2012 |
| Title |
Gettys06_CON_LIVER3 |
| Sample type |
RNA |
| |
|
| Source name |
liver, CON diet
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Fischer 344
gender: Male
tissue: Liver
|
| Treatment protocol |
Livers flash frozen in liquid nitrogen immediately after dissection; stored at -80C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted using TriReagent (Molecular Research Center, Inc. Cincinnati, OH), with added purification of the aqueous layer using Qiagen Rneasy MiniKit. Concentration and quality of RNA assessed using Agilent Bioanalyzer.
|
| Label |
DIG-UTP
|
| Label protocol |
1 ug of total RNA was transcribed to DIG-labeled cRNA using Applied Biosystems Chemiluminescent RT-IVT kit v2.0
|
| |
|
| Hybridization protocol |
10 ug of fragmented DIG-labeled cRNA used for array hybridization according to Applied Biosystems protocol
|
| Scan protocol |
Chemiluminescence detection, imaging, and image analysis performed according to Applied Biosystems protocols and using the 1700 Chemiluminescent Microarray Analyzer Software v 1.0.3
|
| Data processing |
quantile-quantile normalization (www.bioconductor.org)
|
| |
|
| Submission date |
Apr 08, 2011 |
| Last update date |
Apr 10, 2012 |
| Contact name |
Kirsten P Stone |
| E-mail(s) |
kirsten.stone@pbrc.edu
|
| Organization name |
Pennington Biomedical Research Center
|
| Street address |
6400 Perkins Rd
|
| City |
Baton Rouge |
| ZIP/Postal code |
70808 |
| Country |
USA |
| |
|
| Platform ID |
GPL2996 |
| Series (1) |
| GSE28482 |
Effects of 20 months Dietary Methionine Restriction on Hepatic Gene Expression in Fischer 344 Rats |
|
