|
| Status |
Public on Jul 01, 2011 |
| Title |
SG200Dpit2 4dpi rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
maize seedling leave (leaf 3), 4 days after infection
|
| Organism |
Zea mays |
| Characteristics |
variety: Early Golden Bantam
tissue: leaf
disease state: infection with Ustilago maydisstrain SG200Dpit2
|
| Treatment protocol |
Seedlings were inoculated 7 days after sawing with the respective U. maydis strains, resulting in an infection of the third seedling leaf. Samples were taken 4 days after fungal infection.
|
| Growth protocol |
Maize plants (Early Golden Bantam) were grown in a phytochamber in a 15/9 hour light/dark cycle. Temerature was 28°c and 20°c, relative humudity 40% and 60% during light and dark periods, respectively, with 1 hour ramping for both parameters.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Maize Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using protocol Midi_Euk2V3.
|
| Scan protocol |
GeneChips were scanned on a Affymetrix GSC3000G scanner
|
| Description |
Zea mays after infection with Ustilago maydisstrain SG200Dpit2
|
| Data processing |
Date were analyzed with the GeneChip operating software (GCOS) 1.4 to generate CEL files.
|
| |
|
| Submission date |
Apr 08, 2011 |
| Last update date |
Jul 01, 2011 |
| Contact name |
Gunther Doehlemann |
| E-mail(s) |
g.doehlemann@uni-koeln.de
|
| Phone |
+49-6421-178 530
|
| Organization name |
Max Planck Institue for Terrestiral Microbiology
|
| Department |
Organismic Interactions
|
| Street address |
Karl von Frisch Str.
|
| City |
Marburg |
| State/province |
Hessen |
| ZIP/Postal code |
35041 |
| Country |
Germany |
| |
|
| Platform ID |
GPL4032 |
| Series (1) |
| GSE28479 |
Maize gene expression during infection with Ustilago maydis strains SG200Dpit1 and SG200Dpit2 |
|
