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Sample GSM6947329 Query DataSets for GSM6947329
Status Public on Oct 01, 2023
Title Gene expression of Contol BMSCs
Sample type RNA
 
Source name BMSCs
Organism Homo sapiens
Characteristics cell type: Bone Marrow Stem Cells (BMSCs)
treatment: Contol
Extracted molecule total RNA
Extraction protocol Total RNA was prepared from cells or cell derived extracellular vesicles using miRNeasy Serum/Plasma advanced kit (Qiagen).
Label Cy3
Label protocol For each experiment, 100 ng RNAs were reverse treanscribed and followed by generation of cDNAs. The resulting cDNAs were transcribed and labled with cyanine 3-CTP and the labeled antisense cRNAs were purified using RNAeasy columns (QIAGEN).
 
Hybridization protocol The purified antisense cRNAs were hybridized to Agilent’s 8 × 60,000 microarrays (SurePrint G3 Human CGH Microarray 8x60K V2 ) at 45 °C for 16 hrs.
Scan protocol Hybridized microarray slides were washed and scanned on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting for 8x60k array slides
Description Contol BMSCs
BMSC_ctrl_cell
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
 
Submission date Jan 20, 2023
Last update date Oct 02, 2023
Contact name Inyoul Lee
Organization name Institute for Systems Biology
Street address 401 Terry Ave. North
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL16699
Series (2)
GSE223407 Dual targeted extracellular vesicles regulating oncogenic genes in pancreatic cancer [Human Array]
GSE223409 Dual targeted extracellular vesicles regulating oncogenic genes in pancreatic cancer

Data table header descriptions
ID_REF
VALUE Quantile normalized gMean signal intensity (log2)

Data table
ID_REF VALUE
1 13.72353461
2 5.14190718
3 5.760948064
4 10.47510534
5 7.149499923
6 5.110002803
7 5.401646416
8 6.290732035
9 13.60110135
10 7.337003116
11 5.400239751
12 6.793557168
13 6.619705374
14 5.413567456
15 5.367921501
16 5.286518396
17 7.101960968
18 7.069679291
19 7.294594229
20 10.04625219

Total number of rows: 62976

Table truncated, full table size 1089 Kbytes.




Supplementary file Size Download File type/resource
GSM6947329_253949452012_1_4_BMSC_ctrl_cell.txt.gz 7.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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