|
| Status |
Public on Jun 13, 2011 |
| Title |
Schizophrenia ped016 sp061-50K Hind |
| Sample type |
genomic |
| |
|
| Source name |
Schizophrenia ped016 sp061
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: peripheral blood
pedigree_id: 14
individual: father
gender: male
affection _status: healthy
|
| Treatment protocol |
None
|
| Growth protocol |
None
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from peripheral blood using standard phenol/chloroform extraction. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer, PicoGreen and agarose gel.electrophoresis.
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
| Description |
sp061-50K Hind
Hybridized to 50K_Hind
|
| Data processing |
CEL files were processed by manufacture's protocol. The software used to generate the SNP calls is GeneChip DNA Analysis Software (GDAS) v3.0. Matrix tables for the genetic programs were produced. Genotype data were analyzed with the GeneSpring GT (Varia) 2.0 software package developed by Agilent Technologies (Santa Clara, CA). Transmission disequilibrium test was performed using the R program (http://www.r-project.org).
|
| |
|
| Submission date |
Mar 13, 2011 |
| Last update date |
Jun 13, 2011 |
| Contact name |
Kazuo Yamada |
| E-mail(s) |
k_yamada@brain.riken.jp
|
| Phone |
81-48-467-5946
|
| Fax |
81-48-467-7462
|
| Organization name |
RIKEN Brain Science Institute
|
| Lab |
Lab. for Molecular Psychiatry
|
| Street address |
2-1 Hirosawa
|
| City |
Wako-city |
| State/province |
Saitama |
| ZIP/Postal code |
351-0198 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13283 |
| Series (1) |
| GSE27923 |
Affymetrix SNP array data for Japanese schizophrenia samples |
|
