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Status |
Public on Apr 08, 2011 |
Title |
BIO588361 |
Sample type |
RNA |
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|
Source name |
siRNA COP1.JUN scanned on 11/03/10
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Organism |
Homo sapiens |
Characteristics |
cell line: LNCap sirna transfection: siRNA COP1.JUN
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Treatment protocol |
siRNA oligonucleotides (Dharmacon) were transfected with Lipofectamine2000 and LipofectamineRNAiMax (Invitrogen) into LNCaP cells. Cell lysates were prepared 48h after siRNA transfection.
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Growth protocol |
LNCap cells were cultured overnight in serum-free medium.
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Extracted molecule |
total RNA |
Extraction protocol |
Total cellular RNA was prepared with a RNeasy Plus kit (Qiagen) and subject to DNAse I digestion.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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Hybridization protocol |
HGU 133 plus 2.0 arrays were hybridized according to the Affymetrix protocol
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Scan protocol |
HGU 133 plus 2.0 arrays were scanned according to the Affymetrix protocol
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Data processing |
Affymetrix HGU 133 plus 2.0 intensity signal CEL files were processed using the RMA algorithm as implemented in the 'affy' package from Bioconductor.
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Submission date |
Mar 11, 2011 |
Last update date |
Apr 08, 2011 |
Contact name |
Jinfeng Liu |
E-mail(s) |
liu.jinfeng@gene.com
|
Organization name |
Genentech Inc.
|
Department |
Bioinformatics and Computational Biology
|
Street address |
1 DNA Way, MS93
|
City |
South San Francisco |
State/province |
CA |
ZIP/Postal code |
94080 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE27914 |
Expression data from LNCap cell line treated with COP1 (RFWD2), ETV1, and JUN siRNAs |
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