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Sample GSM6761372 Query DataSets for GSM6761372
Status Public on Nov 29, 2022
Title 13_Stroma
Sample type protein
 
Source name TNBC
Organism Homo sapiens
Characteristics disease state: TNBC
roi name: 13
segment name: Stroma
surface area: 109102.621
nuclei count: 612
qc flags: --
patient: TN28
age: 57
grade: 3
t: 1c
n: 2
mets post surgery (months): 24
followup: 1491
status: 1
reponse: PD
metastasis: liver, lung, bone
Extracted molecule protein
Extraction protocol Clinical FFPE samples on individual slides were obtained with appropriate ethics approval
Label NA
Label protocol DSP experiments were performed as per manufacturers DSP Slide Prep User Manual instructions. Slides were deparafinised, antigen retrieval by HIER, and overnight staining with antibody cocktail as per DSP Slide Prep User Manual. Slides were images and samples collected on DSP instrument
 
Hybridization protocol DSP eluates were hybridised as per manufacturers instructions in nCounter Readout User Manual.
Scan protocol Hybridised samples were loaded into Nanostring prep station and processed as per DSP Slide Prep User Manual. N counter was performed with default parameters, and counts from Ncounter uploaded back to DSP instrument
Data processing Counts were aligned and ERCC corrected automatically on the DSP instrument. This QC data was exported for analysis.
 
Submission date Nov 29, 2022
Last update date Nov 29, 2022
Contact name Arutha Kulasinghe
E-mail(s) arutha.kulasinghe@uq.edu.au
Organization name University of Queensland
Street address 37 kent st
City brisbane
ZIP/Postal code 4102
Country Australia
 
Platform ID GPL32896
Series (1)
GSE218973 Spatial Profiling Identifies Prognostic Features of Response to Adjuvant Therapy in Triple Negative Breast Cancer (TNBC)

Data table header descriptions
ID_REF
VALUE norm signal

Data table
ID_REF VALUE
CD11c 8.208213011
CD4 7.783973799
Ki-67 7.302856137
PD-1 6.311947329
PD-L1 3.459791767
HLA-DR 8.83598226
Beta-2-microglobulin 7.304672355
CD3 7.284205469
PanCk 8.910267923
CD68 9.545413398
CTLA4 4.365375599
CD20 5.927424365
SMA 15.65679852
CD56 7.620910326
CD45 9.532405953
CD8 8.172299263
Fibronectin 12.36436123
GZMB 8.544985467
NF1 5.443208544
Cl_Cas9 10.88024257

Total number of rows: 62

Table truncated, full table size 1 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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