|
| Status |
Public on Nov 29, 2022 |
| Title |
2_Stroma |
| Sample type |
protein |
| |
|
| Source name |
TNBC
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: TNBC
roi name: 2
segment name: Stroma
surface area: 201167.1868
nuclei count: 1459
qc flags: --
patient: TN4
age: 42
grade: 3
t: 1c
n: 1
mets post surgery (months): NA
followup: 3137
status: 0
reponse: CR
metastasis: NA
|
| Extracted molecule |
protein |
| Extraction protocol |
Clinical FFPE samples on individual slides were obtained with appropriate ethics approval
|
| Label |
NA
|
| Label protocol |
DSP experiments were performed as per manufacturers DSP Slide Prep User Manual instructions. Slides were deparafinised, antigen retrieval by HIER, and overnight staining with antibody cocktail as per DSP Slide Prep User Manual. Slides were images and samples collected on DSP instrument
|
| |
|
| Hybridization protocol |
DSP eluates were hybridised as per manufacturers instructions in nCounter Readout User Manual.
|
| Scan protocol |
Hybridised samples were loaded into Nanostring prep station and processed as per DSP Slide Prep User Manual. N counter was performed with default parameters, and counts from Ncounter uploaded back to DSP instrument
|
| Data processing |
Counts were aligned and ERCC corrected automatically on the DSP instrument. This QC data was exported for analysis.
|
| |
|
| Submission date |
Nov 29, 2022 |
| Last update date |
Nov 29, 2022 |
| Contact name |
Arutha Kulasinghe |
| E-mail(s) |
arutha.kulasinghe@uq.edu.au
|
| Organization name |
University of Queensland
|
| Street address |
37 kent st
|
| City |
brisbane |
| ZIP/Postal code |
4102 |
| Country |
Australia |
| |
|
| Platform ID |
GPL32896 |
| Series (1) |
| GSE218973 |
Spatial Profiling Identifies Prognostic Features of Response to Adjuvant Therapy in Triple Negative Breast Cancer (TNBC) |
|
