|
| Status |
Public on Dec 12, 2022 |
| Title |
LCS7711_QNZ_C_1_Clean_Data [T-Ctr-1] |
| Sample type |
SRA |
| |
|
| Source name |
CD8+T cells from Balb/c
|
| Organism |
Mus musculus |
| Characteristics |
strain: Balb/c
cell type: sorted CD8+T cells
treatment: transfected with control vector
|
| Treatment protocol |
T cells were transfected with a lentivirus containing a control vector or an FGL2-blocking scFv vector. Cells were spun down at 1500×g for 2 h with 8 μg/mL polybrene (MilliporeSigma) in RPMI medium in a retronectin (T100B; Takara)-coated 24-well plate. T cells were used for in vivo or in vitro experiments 2 to 4 days after transfection.
|
| Growth protocol |
Mouse T cells were isolated from the spleens and lymph nodes of Balb/c, Mouse T cells and human T cells were activated with Dynabeads Mouse T-Activator CD3/CD28 (0077118, Gibco).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After 72 h coculture of T cells with DBT cells at a ratio of 4:1, T cells were collected and stained with anti-CD4 and anti-CD8 antibodies for 30 minutes at RT, then live CD8+ T cells were sorted with a FACSAria III flow cytometer (BD Biosciences). After spindown, cell pellets were frozen and shipped on dry ice to LC Sciences for total RNA extraction and total RNA NGS services.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Paired-end reads from fastq files were aligned and quantified with Salmon (v. 0.14.1) in default setting.
Assembly: GRCm38
Supplementary files format and content: Matrix table with raw gene counts for every gene and every sample
|
| |
|
| Submission date |
Nov 23, 2022 |
| Last update date |
Dec 12, 2022 |
| Contact name |
Shan Jiang |
| E-mail(s) |
sj3195@cumc.columbia.edu
|
| Organization name |
Columbia University Irving Medical Center
|
| Department |
Taub Institute for Research on Alzheimer’s Disease and the Aging Brain
|
| Street address |
710 W 168th St
|
| City |
New York |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE218639 |
RNAseq data of T-Ctr vs T-anti-FGL2 |
|
| Relations |
| BioSample |
SAMN31852550 |
| SRA |
SRX18364030 |
