GEO help: Mouse over screen elements for information.
Status
Public on Sep 26, 2005
Title
Control replicate 7
Sample type
RNA
Source name
Mouse heart
Organism
Mus musculus Characteristics
Control, untreated
Extracted molecule
total RNA
Extraction protocol
Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
Label
biotin
Label protocol
Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Control, untreated
Data processing
Robust Multichip Analysis (RMA)
Submission date
Aug 05, 2005
Last update date
Sep 26, 2005
Contact name
Norman Buroker
E-mail(s)
Norman.Buroker@seattlechildrens.org Organization name
Seattle Children's Hospital
Lab
Portman
Street address
4800 Sand Point Way N.E.
City
Seattle
State/province
WA
ZIP/Postal code
98105
Country
USA
Platform ID
GPL1261 Series (1)
GSE3067 Resistance to thyroid hormone (RTH) and PPARa activation
Data table header descriptions ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
277.8
P
0.000856
AFFX-BioB-M_at
445.8
P
0.000225
AFFX-BioB-3_at
207.3
P
0.000445
AFFX-BioC-5_at
650
P
0.000095
AFFX-BioC-3_at
575.7
P
0.000044
AFFX-BioDn-5_at
729
P
0.000044
AFFX-BioDn-3_at
3627.9
P
0.000081
AFFX-CreX-5_at
8750.8
P
0.000044
AFFX-CreX-3_at
9934.7
P
0.000044
AFFX-DapX-5_at
10664.8
P
0.000044
AFFX-DapX-M_at
14001.9
P
0.000044
AFFX-DapX-3_at
15681.1
P
0.000044
AFFX-LysX-5_at
18.2
A
0.574038
AFFX-LysX-M_at
7.4
A
0.916408
AFFX-LysX-3_at
22
A
0.39692
AFFX-PheX-5_at
2.7
A
0.814869
AFFX-PheX-M_at
2
A
0.993146
AFFX-PheX-3_at
45.8
A
0.262827
AFFX-ThrX-5_at
7.3
A
0.937071
AFFX-ThrX-M_at
1.7
A
0.910522
45101 1205 Kbytes .Supplementary data files not provided
