|
| Status |
Public on Oct 04, 2023 |
| Title |
D4 |
| Sample type |
RNA |
| |
|
| Source name |
plasma exosomes of patients with CSVD
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: circulating plasma exosomes
|
| Treatment protocol |
Exosome was isolated using total exosome isolation (from plasma) reagent (cat no.4484451, Thermo Fisher Scientific, Inc.).Store the isolated exosomes in a refrigerator at -80 °C pending RNA extraction.
|
| Growth protocol |
All subjects had fasting venous blood drawn within 24h of admission, and the blood specimens were immediately centrifuged and stratified (300 g, 4°C, 10 min). The upper layer of plasma was aspirated, diluted to 10 ml with PBS buffer, then stratified by high-speed centrifugation (2000 g, 4°C, 10 min), and the sediment at the bottom of the tube was discarded. The collected plasma was stored in a refrigerator at -80 ℃ pending extraction of exosomes.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA from exosomes was performed according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip miRNA 4.0 Array in GeneChip Hybridization Oven 645. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Data processing |
The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Nov 13, 2022 |
| Last update date |
Oct 04, 2023 |
| Contact name |
Kejin Gao |
| E-mail(s) |
2020026122@qdu.edu.cn
|
| Organization name |
The Affiliated Hospital of Qingdao University
|
| Street address |
NO.59 hai er Rd, Qingdao, China
|
| City |
QingDao |
| State/province |
Shan Dong |
| ZIP/Postal code |
266061 |
| Country |
China |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE217872 |
A retrospective clinical study to explore diagnostically significant markers in circulating exosomes of patients with cerebral small vessels disease (CSVD) |
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